Pregled bibliografske jedinice broj: 1243093
The effects of maternal cigarette smoking on cadmium and lead levels, miRNA expression and biochemical parameters across the feto-placental unit
The effects of maternal cigarette smoking on cadmium and lead levels, miRNA expression and biochemical parameters across the feto-placental unit // Heliyon, 8 (2023), e12568, 15 doi:10.1016/j.heliyon.2022.e12568 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1243093 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The effects of maternal cigarette smoking on
cadmium and lead levels, miRNA expression and
biochemical parameters across the feto-placental
unit
Autori
Sekovanić, Ankica ; Dorotić, Adrijana ; Pašalić, Daria ; Orct, Tatjana ; Kljaković-Gašpić, Zorana ; Sulimanec Grgec, Antonija ; Stasenko, Sandra ; Mioč, Tatjana ; Piasek, Martina ; Jurasović, Jasna
Izvornik
Heliyon (2405-8440) 8
(2023);
E12568, 15
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
miR-146a ; miR-16 ; miR-21 ; Placenta ; Postpartum women ; Urate
Sažetak
Several miRNAs have been previously identified to be associated with cigarette smoke and/or the toxic metals cadmium (Cd) and lead (Pb). The aim of this study was to investigate the associations of maternal cigarette smoking with cadmium (Cd) and lead (Pb) levels, candidate miRNA expression and biochemical parameters across the feto- placental unit. miRNAs were isolated according to protocols provided by manufacturer from 72 healthy postpartum women using Qiagens’ kits based on phenol/guanidine samples lysis and silica- membrane purification of total RNA. Candidate miRNAs (miR-1537, miR-190b, miR-16, miR-21, and miR-146a) were quantified by real-time PCR. Biochemical parameters were analyzed in plasma samples by standardized and harmonized enzymatic methods using appropriate calibrators, while CRP was determined by immunoturbidimetric method. Concentration of Cd and Pb in whole blood and placenta samples were measured by inductively coupled plasma mass spectroscopy. Cd levels in smokers were higher in all of the analyzed compartments of the feto-placental unit, Pb in maternal blood and placenta than non-smokers. Smokers also had a higher expression of miR-16 in maternal and miR-146a in cord plasma, and lower expression of miR-21 in the placenta in comparison to non-smokers. Urate concentrations in the maternal plasma of smokers were lower than this value in nonsmokers. The study has demonstrated that maternal smoking was associated with toxic metals (Cd and Pb) levels, urate concentration and alteration of miRNA expression. Given that the effects of maternal smoking on miRNA expression are inadequate, all compartments of the feto- placental unit should be analyzed to obtain a complete picture. This paper is the first to report on the results of expression of cellular and circulating miRNAs simultaneously in maternal and fetal compartments and in the placenta.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-1998 - Procjena svakodnevne izloženosti metalima i osobne osjetljivosti majke kao čimbenika razvojnoga podrijetla zdravlja i bolesti (METALORIGINS) (Piasek, Martina; Jurasović, Jasna, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Medicinski fakultet, Zagreb
Profili:
Adrijana Dorotić
(autor)
Tatjana Orct
(autor)
Martina Piasek
(autor)
Jasna Jurasović
(autor)
Sandra Stasenko
(autor)
Antonija Sulimanec Grgec
(autor)
Zorana Kljaković Gašpić
(autor)
Daria Pašalić
(autor)
Ankica Sekovanić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus