Pregled bibliografske jedinice broj: 1241429
Microfiltration affects mycotoxin recovery
Microfiltration affects mycotoxin recovery // Abstracts of the international symposium Power of Fungi and Mycotoxins in the Midst of Climate Change (PoFMy)
Koprivnica, Hrvatska, 2022. str. 33-33 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1241429 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Microfiltration affects mycotoxin recovery
Autori
Kovač Tomas, Marija ; Mijatović, Anto ; Rot, Tomislav ; Bulaić, Mateja ; Šarkanj, Bojan ; Kovač, Tihomir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstracts of the international symposium Power of Fungi and Mycotoxins in the Midst of Climate Change (PoFMy)
/ - , 2022, 33-33
Skup
Power of Fungi and Mycotoxins in the Midst of Climate Change (PoFMy)
Mjesto i datum
Koprivnica, Hrvatska, 16.09.2022. - 17.09.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
liquid chromatography-tandem mass spectrometry ; multi-mycotoxins ; public health ; risk assessment ; sample preparation
Sažetak
Frequent multi-mycotoxin occurrences in various foods, alongside adverse health effects and legislative requirements, demand constant and continuous monitoring to properly assess the level of mycotoxin contamination. Sensitive and unambiguous quantification of multiple analytes requires state-of-the-art analytics, such as liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). To protect the instrument from small particles while reducing the matrix effect, many LC-MS/MS methods employ membrane filtration during sample preparation. However, when membrane filtration is used, possible adsorptive effects at membrane filters should also be considered, as they could prevent the accurate determination of mycotoxin (co-)occurrence and the real risk assessment for public health. In this study, five different material types of syringe filters, i.e. nylon (NY), polytetrafluoroethylene (PTFE), polyethersulfone (PES), mixed cellulose ester (MCE), and cellulose acetate (CA), were used to assess the impact of microfiltration on mycotoxin recovery. All 11 of the legislatively regulated mycotoxins were selected for the investigation, including aflatoxins B1, B2, G1 and G2, deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, and ochratoxin A. The combined mycotoxin standard solution was prepared in concentrations 0.25-50 ng/mL using acetonitrile/water/formic acid (49.5/49.5/1, v/v/v). This multi-mycotoxin solution was filtrated using different syringe filters where the first three filtrate drops and the following filtrate were collected separately into vials and injected in triplicates into a LC-MS/MS system and analysed using a validated instrumental method. The recovery for each mycotoxin was calculated comparing the average area of the filtered and unfiltered standard solution and expressed in percentage. The results revealed that PTFE filters have the least impact on mycotoxin loss during filtration, while commonly used NY filters could affect the recoveries of certain mycotoxins if not properly used. A decrease in the recoveries of almost all mycotoxins was observed in the first three nylon filter drops, especially for aflatoxin B1 and zearalenone (up to 30 %), while the following filtrate contained a satisfying amount of all of the tested mycotoxins, when compared to the unfiltered solution. Therefore, for accurate mycotoxin determination, the first few filtrate drops should be discarded, as they saturate membrane filter materials, once again pointing to the importance of a proper method development procedure.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija, Prehrambena tehnologija
POVEZANOST RADA
Ustanove:
Prehrambeno-tehnološki fakultet, Osijek,
Sveučilište Sjever, Koprivnica