Naslov
Comparison of a novel Alifax platform for a rapid screening of multi drug resistant organisms (MDRO) with classical phenotypic method in a University Hospital of Split, Croatia

Autori
Novak, Anita ; Marinović, Jelena ; Rubic, Zana ; Goic-Barisic, Ivana ; Radic, Marina ; Tonkic, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Skup
12. Hrvatski kongres kliničke mikrobiologije ; 9. Hrvatski kongres o infektivnim bolestima (CROCMID 2019)

Mjesto i datum
Split, Hrvatska, 24.10.2019. - 27.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
multi-drug resistance, diagnostic

Sažetak
Background and aim: Infections caused by MDRO are difficult to treat and associated with poor outcomes for patients. Therefore, prevention of these infections is essential. Aim of the study was to compare the performance of a new rapid method for the detection of MDRO with cultivation on selective media. Material/methods: On two selected days, Intensive Care Unit's patients in University Hospital of Split, Croatia, were screened for the presence of methicillin-resistant Staphylococcus aureus (MRSA) and Extended Spectrum ß-Lactamase (ESBL)-producing Enterobacteriaceae. Twenty one axillary swabs, twenty nose swabs and 19 rectal swabs were collected. Specimens were taken in duplicate, one for rapid and the other one for a classical screening. Classic method was performed by cultivation on solid and liquid selective media and incubated at 37°C for 24 h. On the second day, all liquid media were subcultivated and incubated for another 24 h. All isolated strains were identified with Vitek 2 system (additional 24 h). Rapid screening method, based on laser light scattering technology, (HB&L Uroquattro, Alifax) was performed according to the manufacturer’s instructions. For that purpose, HB&L MRSA and HB&L ESBL/AmpC kits were used. The growth phases of bacteria were monitored, providing real time growth curves. The presence of MDRO was detected in 6.5 hours. Results: With conventional method, 10 positive ESBL stains from 10 different rectal specimens were isolated on ESBL media. Exactly the same specimens were positive for ESBL grow by rapid Alifax method. On selective MRSA media, 4 strains of Coagulase negative methicillin resistant Staphylococcus sp, 5 strains of MDR Acinetobacter baumannii and 2 MRSA strains were cultured. Rapid method detected 11 positive specimens. Conclusions: High concordance of results obtained with classical and rapid method is encouraging. A novel method is fast and reliable and it can be recommended for a routine MDRO screening due to it’s ability to identify other şpecies resistant to methicillin (beside MRSA), such as Acinetobacter baumannii. Colonized patients could be isolated at the same day while patients transferred from another hospitals with negative screening result, could be safely admitted to the hospital wards. However, these results should be confirmed on bigger sample size.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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