Naslov
Occurrence and characterization of Clostridioides difficile infection (CDI) in a University Hospital of Split (UHS)

Autori
Lozina, Marijeta ; Novak, Anita ; Rubic, Zana ; Goic-Barisic, Ivana ; Radic, Marina ; Marinovic, Jelena ; Tonkic, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
12. Hrvatski kongres kliničke mikrobiologije ; 9. Hrvatski kongres o infektivnim bolestima (CROCMID 2019)

Mjesto i datum
Split, Hrvatska, 24.10.2019. - 27.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
C. difficile

Sažetak
Background and aim: Clostridioides difficile (CD) is the most important infective cause of healthcare-associated diarrhoea worldwide. Aim of the present study is epidemiological and microbiological characterization of Clostridioides difficile infection (CDI) in a University Hospital of Split. Methods: During the study period (January to December 2017), data base of the Department of Clinical Microbiology was retrospectively examined. All patients with a first episode of CDI were enrolled in the study. Demographic data and microbiological characteristics of C. difficile were analyzed. For CDI diagnostic, two-step algorithm was used. Stool samples were tested for the presence of glutamate dehydrogenase (GDH) and toxins A/B, using immunoassay card (CoproStrip C. difficile GDH + Toxin A/B, Savyon Diagnostics, Israel). GDH positive, ToxinA/B negative strains were further tested with molecular tests (llumigene MeridianBioscience, Italy). Results: A total of 4126 stool samples were tested for a bacterial infection and 519 pathogens were found. Among them, C. difficile was the most frequent (71%), following with Campylobacter species (13%), enteropathogenic Escherichia coli (9%) and Salmonella enterica (7%) (Fig.1). Primary C. difficile infection occurred in 370 patients, while recurrent infection had 15% of them. Majority of infections (64%) had hospital occurrence. Among hospitalized patients, majority were located at the Department for internal medicine (40%), Intensive care units (28%) and Department for infectious diseases (17%). Proportion of female patients (59%) was significantly higher in comparison to male (Fig.2). Significantly more patients were older than 65 years (62%). Active toxin production was recorded in 291 samples (only toxin A in 9%, only toxin B in 5%, and both toxins in 65%). In the remaining 21% of positive samples, toxigenic C. difficile strain without active production of toxins was detected. Conclusion: Clostridioides difficile is the most common bacterial cause of diarrhea in University Hospital of Split. CDI occurred mostly among hospitalized patients older than 65 and female patients were far more affected. Majority of CDI were caused by strains with active toxin production.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA