Naslov
Campylobacter spp. in Shellfish in Croatia

Autori
Humski, Andrea ; Ječmenica, Biljana ; Džafić, Natalija ; Brlek Gorski, Diana ; Šimpraga, Borka ; Krstulović, Fani ; Amšel Zelenika, Tajana ; Jurinović, Luka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CHRO 2022, Abstract Booklet / Tang, Yuanyue ; Wang, Biyong ; Xiang, Hui ; Zhang, Ying ; Zhang, Suxing - Yangzhou, China, 2022, 116-117

Skup
21st International Workshop on Campylobacter, Helicobacter and Related Organism

Mjesto i datum
Yangzhou, China, 14.11.2022. - 19.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Campylobacter jejuni ; Campylobacter lari ; bivalve molluscs ; MLST

Sažetak
The aim of this study was to assess the presence of thermotolerant Campylobacter species in shellfish production and harvesting areas of the Istrian aquatory (North Adriatic Sea), regarding the growing conditions (in terms of location and sea temperature) and shellfish species, as factors influencing increased public health risk when consuming shellfish. The study included samples of the most economically important species of live bivalve molluscs: mussels (Mytilus galloprovincialis), oysters (Ostrea edulis) and queen scallops (Aequipecten opercularis) that were collected in a period from August to October 2021, on a weekly basis at nine locations of the Istrian County, Croatia. Isolation of Campylobacter was done according to standard ISO 10272-1:2017 method, and species were identified using multiplex PCR. Isolates identified as C. jejuni and C. lari were genotyped using multilocus sequence typing (MLST). Among 108 examined samples of bivalve molluscs, mussels dominated and were the only ones found positive for the presence of Campylobacter (25.6%). The majority of Campylobacter positive samples, 85% of them (n = 17), were detected when the sea temperature was between 20 °C and 27 °C. In total, 19 C. lari and one C. jejuni strains were isolated. C. lari isolates found in this study belong to 13 sequence types (STs), and 9 of them are newly described in this research. Two out of the four previously described C. lari STs that were found in this study were previously detected in human stool. The only C. jejuni isolate was found to be sequence type 1268, which belongs to ST-1275 clonal complex that is almost exclusively found in seabirds but can sporadically cause infection in humans. Based on the obtained results, introducing surveillance of thermotolerant Campylobacter in shellfish in the Republic of Croatia is advised as an improvement for public health safety.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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