Pregled bibliografske jedinice broj: 1239251
Prevalence and Characterization of Shiga Toxin Producing Escherichia coli Isolated from Animal Feed in Croatia
Prevalence and Characterization of Shiga Toxin Producing Escherichia coli Isolated from Animal Feed in Croatia // Microorganisms, 10 (2022), 9; 1-13 doi:10.3390/microorganisms10091839 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1239251 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Prevalence and Characterization of Shiga Toxin Producing Escherichia coli Isolated from Animal Feed in Croatia
Autori
Sokolovic, Marijana ; Šimpraga, Borka ; Amšel-Zelenika, Tajana ; Berendika, Marija ; Krstulović, Fani
Izvornik
Microorganisms (2076-2607) 10
(2022), 9;
1-13
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Escherichia coli ; STEC ; feed ; poultry ; stx ; eae
Sažetak
A survey on prevalence and number of Shiga toxin-producing Escherichia (E.) coli (STEC) in animal feed was carried out over a period of nine years in the Republic of Croatia. A total of 1688 feed samples were collected from feed factories and poultry farms. Analysis included two standard procedures: sample enrichment and (a) immunomagnetic separation and plating on two selective media ; or (b) plating on two selective media. Confirmation of STEC included morphological examination, biochemical tests, serotyping, and polymerase chain reaction. Morphological and biochemical characterization revealed 629 E. coli strains. Further serological screening method revealed 78 STEC and EPEC serotypes, while only 27 strains were confirmed as STEC with PCR. All positive samples (1.6%) originated from poultry farms and contained combination of virulence genes: eaeA, stx1, and/or stx2. Since the presence of stx (especially stx2) and eae are identified as risk factors for development of severe diseases in humans, results of this survey indicate that avian sources of STEC infections might be one of those “undefined sources” of human illnesses. Further research is necessary for evaluation of risks posed by contaminated feed, poultry, and environment.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Veterinarska medicina
POVEZANOST RADA
Ustanove:
Hrvatski veterinarski institut, Zagreb
Profili:
Borka Šimpraga
(autor)
Marijana Sokolovic
(autor)
Tajana Amšel Zelenika
(autor)
Marija Berendika
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus