Pregled bibliografske jedinice broj: 1238297
Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation
Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation // Lipid Bilayers at ESS - BESS
Lund, 2022. str. 1-1 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1238297 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Properties of spin-coated
phosphatidylcholine/sphingomyelin/cholesterol
lipid
films and its effect on giant unilamellar vesicle
electroformation
Autori
Mardešić, Ivan ; Boban, Zvonimir ; Raguž, Marija
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
Lipid Bilayers at ESS - BESS
Mjesto i datum
Lund, Švedska, 13.06.2022. - 15.06.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
GUVs ; electroformation ; cholesterol ; sphingomyelin ; phosphatidylcholine ; film thickness ; AFM
Sažetak
Lipid film deposition is an important step in production of artificial vesicles using the electroformation method. Traditional protocols obtain lipid films by simply dropping the chloroform dissolved lipids onto the surface. However, films obtained in such a manner are very inhomogeneous and regions of different thicknesses appear. To address this problem, alternative deposition techniques such as spin-coating have been attempted. We used atomic force microscopy and X- ray reflectometry to analyze the spin-coated phosphatidylcholine/ sphingomyelin/cholesterol lipid films. After utilizing these films for giant unilamellar vesicle electroformation an analysis of obtained fluorescence microscopy images was performed. The optimal thickness for two phosphatidylcholine/ sphingomyelin/cholesterol molar mixtures, 1/1/1 and 1/1/4, was found to be around 30 nm.
Izvorni jezik
Engleski
Znanstvena područja
Fizika, Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
IP-2019-04-1958 - Otkrivanje organizacije i funkcije kolesterolove domene u plasma membrani fibroznih stanica leće oka uz primjenu fluorescentnih metoda (BioPhyCBD) (Raguž, Marija, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Medicinski fakultet, Split