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Pregled bibliografske jedinice broj: 1238297

Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation


Mardešić, Ivan; Boban, Zvonimir; Raguž, Marija
Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation // Lipid Bilayers at ESS - BESS
Lund, 2022. str. 1-1 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1238297 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation

Autori
Mardešić, Ivan ; Boban, Zvonimir ; Raguž, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Lipid Bilayers at ESS - BESS

Mjesto i datum
Lund, Švedska, 13.06.2022. - 15.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
GUVs ; electroformation ; cholesterol ; sphingomyelin ; phosphatidylcholine ; film thickness ; AFM

Sažetak
Lipid film deposition is an important step in production of artificial vesicles using the electroformation method. Traditional protocols obtain lipid films by simply dropping the chloroform dissolved lipids onto the surface. However, films obtained in such a manner are very inhomogeneous and regions of different thicknesses appear. To address this problem, alternative deposition techniques such as spin-coating have been attempted. We used atomic force microscopy and X- ray reflectometry to analyze the spin-coated phosphatidylcholine/ sphingomyelin/cholesterol lipid films. After utilizing these films for giant unilamellar vesicle electroformation an analysis of obtained fluorescence microscopy images was performed. The optimal thickness for two phosphatidylcholine/ sphingomyelin/cholesterol molar mixtures, 1/1/1 and 1/1/4, was found to be around 30 nm.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
IP-2019-04-1958 - Otkrivanje organizacije i funkcije kolesterolove domene u plasma membrani fibroznih stanica leće oka uz primjenu fluorescentnih metoda (BioPhyCBD) (Raguž, Marija, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Medicinski fakultet, Split

Profili:

Avatar Url Marija Raguž (autor)

Avatar Url Zvonimir Boban (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada

Citiraj ovu publikaciju:

Mardešić, Ivan; Boban, Zvonimir; Raguž, Marija
Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation // Lipid Bilayers at ESS - BESS
Lund, 2022. str. 1-1 (poster, međunarodna recenzija, sažetak, znanstveni)
Mardešić, I., Boban, Z. & Raguž, M. (2022) Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation. U: Lipid Bilayers at ESS - BESS.
@article{article, author = {Marde\v{s}i\'{c}, Ivan and Boban, Zvonimir and Ragu\v{z}, Marija}, year = {2022}, pages = {1-1}, keywords = {GUVs, electroformation, cholesterol, sphingomyelin, phosphatidylcholine, film thickness, AFM}, title = {Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation}, keyword = {GUVs, electroformation, cholesterol, sphingomyelin, phosphatidylcholine, film thickness, AFM}, publisherplace = {Lund, \v{S}vedska} }
@article{article, author = {Marde\v{s}i\'{c}, Ivan and Boban, Zvonimir and Ragu\v{z}, Marija}, year = {2022}, pages = {1-1}, keywords = {GUVs, electroformation, cholesterol, sphingomyelin, phosphatidylcholine, film thickness, AFM}, title = {Properties of spin-coated phosphatidylcholine/sphingomyelin/cholesterol lipid films and its effect on giant unilamellar vesicle electroformation}, keyword = {GUVs, electroformation, cholesterol, sphingomyelin, phosphatidylcholine, film thickness, AFM}, publisherplace = {Lund, \v{S}vedska} }




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