Pregled bibliografske jedinice broj: 1238296
Optimization of giant unilamellar vesicle electroformation from binary and ternary lipid mixtures with high cholesterol content
Optimization of giant unilamellar vesicle electroformation from binary and ternary lipid mixtures with high cholesterol content // Lipid Bilayers at ESS - BESS
Lund, 2022. str. 1-1 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1238296 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Optimization of giant unilamellar vesicle
electroformation from binary and ternary lipid
mixtures with high cholesterol content
Autori
Boban, Zvonimir ; Mardešić Ivan ; Raguž Marija
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
Lipid Bilayers at ESS - BESS
Mjesto i datum
Lund, Švedska, 13.06.2022. - 15.06.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
GUVs ; electroformation ; cholesterol ; sphingomyelin ; phosphatidylcholine ; frequency ; voltage ;
Sažetak
Giant unilamellar vesicles are popular model membrane systems in research due to having a size similar to that of eukaryotic cells. Electroformation is the method most commonly used for their production. However, due to a large number of parameters, their values often have to be adjusted when working with new mixtures. Here we used phosphatidylcholine/cholesterol and phosphatidylcholine/sphingomyelin/cholesterol lipid mixtures. Fluorescence and atomic force microscopy were utilized to find the optimal values of electrical parameters and lipid film thicknesses. Special attention was given to the effect of different cholesterol contents, with measurements performed for cholesterol concentrations surpassing the phospholipid bilayer saturation threshold. Such high cholesterol contents are of interest to researchers studying the role of cholesterol in fiber cell plasma membranes of the eye lens or in the development of atherosclerosis. We show that increasing the cholesterol concentration decreases the vesicle size, but this effect can be reduced if appropriate electroformation parameters are chosen.
Izvorni jezik
Engleski
Znanstvena područja
Fizika, Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
IP-2019-04-1958 - Otkrivanje organizacije i funkcije kolesterolove domene u plasma membrani fibroznih stanica leće oka uz primjenu fluorescentnih metoda (BioPhyCBD) (Raguž, Marija, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Medicinski fakultet, Split