Pregled bibliografske jedinice broj: 1238278
Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results
Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results // Membranes, 11 (2021), 11; 860, 17 doi:10.3390/membranes11110860 (međunarodna recenzija, članak, znanstveni)
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Naslov
Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results
Autori
Boban, Zvonimir ; Mardešić, Ivan ; Subczynski, Witold Karol ; Raguž, Marija
Izvornik
Membranes (2077-0375) 11
(2021), 11;
860, 17
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
electroformation ; GUVs ; cholesterol ; lipid composition ; lipid deposition ; electrical parameters ; temperature ; electroformation duration ; internal solution ; quantitative analysis
Sažetak
Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of a large number of parameters, different lipid compositions, and internal solutions in order to avoid artifactual results or reproducibility problems. These issues motivated us to write a short review of the most recent methodological developments and possible pitfalls. Additionally, since traditional manual analysis can lead to biased results, we have included a discussion on methods for automatic analysis of GUVs. Finally, we discuss possible improvements in the preparation of GUVs containing high cholesterol contents in order to avoid the formation of artifactual cholesterol crystals. We intend this review to be a reference for those trying to decide what parameters to use as well as an overview providing insight into problems not yet addressed or solved.
Izvorni jezik
Engleski
Znanstvena područja
Fizika
POVEZANOST RADA
Projekti:
IP-2019-04-1958 - Otkrivanje organizacije i funkcije kolesterolove domene u plasma membrani fibroznih stanica leće oka uz primjenu fluorescentnih metoda (BioPhyCBD) (Raguž, Marija, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus