Naslov
The subcellular localization and oligomerization preferences of NME1/NME2 upon radiation-induced DNA damage

Autori
Radić, Martina ; Šoštar, Marko ; Weber, Igor ; Ćetković, Helena ; Slade, Neda ; Herak Bosnar, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th Croatian Microscopy Congress with Internatial Participation : Book of Abstracts / Jelena, Macan ; Goran, Kovačević - Zagreb : Hrvatsko mikroskopijsko društvo ; Institut Ruđer Bošković, 2022, 8-8

ISBN
978-953-7941-41-3

Skup
4th Croatian Microscopy Congress (CMC 2022)

Mjesto i datum
Poreč, Hrvatska, 18.05.2022. - 20.05.2022

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
NME protein family ; NDPK ; DNA damage ; FRET/FLIM ; live-cell imaging

Sažetak
Nucleoside diphosphate kinases (NDPK/NME/Nm23) are housekeeping enzymes responsible for the maintenance of the cellular NTP pool by catalyzing the exchange of terminal phosphate from trinucleotides to dinucleotides through a high-energy phosphohistidine intermediate. At least 80 % of the cytoplasmic NDPK activity is being exerted by NME1/NDPK A and NME2/NDPK B which combine to form isoenzymes (A6, A5B1, …, B6). The NME1/NDPK A and NME2/NDPK B subunits have a variety of additional biological functions, from metastasis suppression and DNA damage repair to proliferation and development. Although eukaryotic NDPKs are active only as hexamers, it is unclear whether other NME functions require the hexameric form and how the isoenzyme composition varies in different cellular compartments. To investigate the effect of DNA damage on the intracellular localization of NME1 and NME2 and the composition of NME oligomers in the nucleus and cytoplasm, we used live-cell imaging and the FRET/FLIM technique. We demonstrated that exogenous NME1 and NME2 proteins colocalize in the cytoplasm of non-irradiated cells and move simultaneously to the nucleus after gamma irradiation. The FRET/FLIM experiments suggest that there is a slight shift in the homomer/heteromer balance between the nucleus and cytoplasm after DNA damage. Overall, our results suggest that NME1 and NME2 engage in mutual functions in the nucleus after irradiation, possibly performing specific functions in their homomeric state.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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