Naslov
Virome Of The Invasive Signal Crayfish And Its Variations Along The Host’s Invasion Range

Autori
Bačnik, Katarina ; Kutnjak, Denis ; Černi, Silvija ; Dragičević, Paula ; Bielen, Ana ; Hudina, Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Slovenian Microbiome Network Symposium Abstract Book / Stopnišek, Nejc ; Janežič, Sandra - Maribor : Nacionalni laboratorij za zdravje, okolje in hrano Maribor, 2022, 50-50

Skup
1st Slovenian Microbiome Network Symposium

Mjesto i datum
Bled, Slovenija, 24.11.2022. - 24.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microbiome ; viruses ; RNA ; sequencing ; Pacifastacus leniusculus

Sažetak
Crayfish are keystone species of freshwater ecosystems and successful invasive species, however, their pathogens, including viruses, remain understudied. The aim of this study was to analyze the virome of one of the most successful invader in European freshwaters, the signal crayfish (Pacifastacus leniusculus), and shed light on the potential differences in viral sequence composition along its invasion range in the Korana River, Croatia. 120 hepatopancreas tissues were dissected from signal crayfish sampled along its invasion range at four sites: upstream invasion front and core and downstream invasion front and core. Total RNA was isolated, depleted of ribosomal RNA and used for high- throughput sequencing. Subsequent bioinformatics analysis based on protein similarity search of de novo assembled contigs identified novel RNA viruses belonging to reo-like, hepe-like, toti-like and picorna-like viral clades. Sequences of novel signal crayfish associated viruses had relatively low levels of similarity to known viral sequences, however they were phylogenetically related to viruses previously associated with crustacean hosts. The patterns of viral reads abundance and calculated nucleotide diversities of the detected viral sequences varied between the sampling locations along the invasion range, which could indicate the possible influence of different factors and processes on signal crayfish virome composition: e.g., the differences in signal crayfish population density and transfer of viruses from the native co-occurring and phylogenetically related crayfish species. The study reveals a high, previously undiscovered diversity of divergent RNA viruses associated with signal crayfish and set foundations for understanding the potential risk of virus transmissions as a result of this invader’s dispersal.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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