Naslov
Ex vivo 7T magnetic resonance imaging of testicular tissue: preliminary results
(Ex vivo 7T magnetic resonance imaging of testicular tissue: preliminary results)

Autori
Planinić, Ana ; Škokić, Siniša ; Ježek, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal für Reproduktionsmedizin und Endokrinologie, Vol. 17, Suppl. 1 (2020) / - Gablitz : Krause und Pachernegg GmbH, 2020, 68-68

Skup
12th International Congress of Andrology ; 11th European Congress of Andrology ; 32nd German Congress of Andrology

Mjesto i datum
Münster, Njemačka; online, 05.12.2020. - 09.12.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
azoospermia ; sperm retrieval ; magnetic resonance imaging

Sažetak
Men with azoospermia who wish to have children undergo testicular sperm extraction (TESE) which has a success rate of only about 60% which means that a significant number of patients are needlessly exposed to risks of the procedure. Our study aimed to assess ex-vivo MRI of testicular tissue, including diffusion-weighted imaging (DWI) as a potential tool in developing a prediction model for sperm retrieval as well as assess the differences between fresh tissue and frozen-thawed tissue. The study included 18 samples of testicular tissue obtained via TESE, 9 that underwent 7T MRI as fresh tissue (group 1) and 9 that were frozen in liquid nitrogen, thawed and then imaged (group 2). All samples were then histologically analyzed. Both groups consisted of two subgroups, samples with sperm (A ; n=5) and samples without sperm (B ; n=4). T1, T2 and ADC values were measured in all samples and values were compared between groups and subgroups. T2 was increased in group 2 compared to group 1 (p=0, 000041) while no statistically significant difference in ADC or T1 was observed between groups. Within group 1, ADC was increased in subgroup B compared to subgroup A and the increase was statistically significant (p=0, 019) while in group 2 the increase was not statistically significant (p=0, 0585). No statistically significant difference in ADC values was observed between subgroups 1A and 2A or 1B and 2B. In conclusion, T2 is significantly increased in frozen and thawed tissue compared to fresh tissue which should be considered when interpreting data and could reflect the effect of freezing and thawing on the consistency and increased water diffusion into the tissue. ADC could be a useful parameter in evaluating fresh and frozen-thawed testicular tissue and predicting sperm retrieval but should be evaluated in a study with larger sample sizes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA