Naslov
PALINDROME-INITAITED GENOME INSTABILITY IN EUKARYOTIC CELLS

Autori
Svetec Miklenić, Marina ; Gatalica, Nikolina ; Svetec, Ivan Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB22 From Science to Knowledge / - , 2022, 139-139

Skup
HDBMB22: From Science to Knowledge

Mjesto i datum
Brela, Hrvatska, 28.09.-01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
palindromes, genome instability, S. cerevisiae

Sažetak
Palindromes in DNA are symmetrical motifs consisting of two inverted repeats, i.e. having the same nucleotide sequence in the two complementary strands when read in the same direction of polarity. The inverted repeats can be completely identical and adjacent to each other which is referred to as a perfect palindrome, or have mismatches and/or a spacer region in between. If a palindrome is long enough, this symmetry allows for intrastrand base pairing to occur and secondary structures can form in dsDNA. Although palindromes are often found as important functional elements of cis-acting regions and protein binding sites in the genomes, they are also known as potentially dangerous elements which can lead to double strand breaks and thus instigate genetic recombination. In turn, this can lead to various rearrangements - deletions, duplications, translocations or amplifications which are all common features of cancer cells. In particular, palindromic amplifications which alter expression levels of certain genes are linked to poor disease outcome since they can make cancer cells more proliferative and more resistant to drugs. The aim of our research effort is to elucidate molecular mechanisms of palindrome recombinogenicity in the eukaryotic genome using yeast Saccharomyces cerevisiae as a model organism. Within this aim, we are pursuing several areas of research: first one is focused on the features of the palindrome sequence (e.g. the relationship between palindrome recombinogenicity and its length as well as the spacer length) ; second, on the physical conditions of the environment (e.g. the influence of growth temperature on palindrome recombinogenicity) ; and third, on the proteins and pathways involved in occurrence and repair of palindrome-initiated double strand break and its potential consequences for the genome stability. Taken together, the knowledge collected in S. cerevisiae can help better understand the underlying causes of palindrome-initated genetic instability in cancer cells.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA