Naslov
Polyphenol bioavailability and modulatory potential on brain antioxidative markers in C57BL/6 mouse

Autori
Đikić, Domagoj ; Balta, Vedran ; Pedisić, Sandra ; Zorić, Zoran ; Padovan, Jasna ; Butorac, Dražan ; Milić, Astrid ; Jurić, Dragan ; Landeka Jurčević, Irena

Izvornik
PERIODICUM BIOLOGORUM (0031-5362) 124 (2022), 1-2; 41-54

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
: Prunus spinosa extract ; polyphenols ; bioapsorption ; neuroprotection

Sažetak
Background and purpose: Prunus spinose L. is a traditionally consumed, recently scientifically reexamined plant. Brain bioavailability and functionality of polyphenols (PPH) of blackthorn flower extract (PSE) was investigated. Materials and methods: C57BL/6 mice received oral daily repeated doses of 25 mg/kg body weight of total PSE polyphenols for 28 days. Brain concentrations of individual polyphenols from PSE were determined by UPLC/MS on 1st, 7th, 14th, 21st, and 28th day. Brain antioxidative defense markers were examined as indicators of functionality after bioaccumulation. Results: A total of 68.7% PPH present in PSE were detected in the brain. Higher (p≤0.05) Cmax/AUClast in the PSE treatment vs. control group was recorded for 59.1% of brain detected compounds, indicating relatively good bioaccumulation in the brain. The highest present compounds in PSE were not necessarily the ones mostly bioapsorbed in the brain. Kaempherols were not significantly distributed, opposite to phenolic acids, quercetins or epicgaloatechin-3- gallate. The compounds with the highest concentrations on 28th day were 4-p- coumaroylquinonic acid, (-)-epicatechin, quercetin-3-O-rutinoside, quercetin–rhamnoside, kaempherol-3-rutinoside and quercetin-3-gucoside. Brain lipid peroxidation (MDA) decreased (p<0.05) on the 21st and 28th day in the PSE group. Increase (p<0.05) in GSH concentration was observed on the 21st and 28th and SOD activity on the 28th day. Catalase activity was unchanged. It could be hypothesized that highest PPH concentration-ratios, caused reduction of lipid peroxidation by radical scavenging and simultaneous induction of glutathione and SOD pathways. Conclusions: Screened compounds could be candidates for examining or creation of brain targeted “neuro-nutriceuticals” polyphenol mixtures.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita, Biotehnologija, Prehrambena tehnologija, Nutricionizam

Napomena
Background and purpose: Prunus spinose L. is a
traditionally consumed, recently scientifically
reexamined plant. Brain bioavailability and
functionality of polyphenols (PPH) of blackthorn
flower extract (PSE) was investigated.
Materials and methods: C57BL/6 mice received oral
daily repeated doses of 25 mg/kg body weight of
total PSE polyphenols for 28 days. Brain
concentrations of individual polyphenols from PSE
were determined by UPLC/MS on 1st,7th, 14th, 21st,
and 28th day. Brain antioxidative defense markers
were examined as indicators of functionality after
bioaccumulation.
Results: A total of 68.7% PPH present in PSE were
detected in the brain. Higher (p≤0.05)
Cmax/AUClast in the PSE treatment vs. control
group was recorded for 59.1% of brain detected
compounds, indicating relatively good
bioaccumulation in the brain. The highest present
compounds in PSE were not necessarily the ones
mostly bioapsorbed in the brain. Kaempherols were
not significantly distributed, opposite to
phenolic acids, quercetins or epicgaloatechin-3-
gallate. The compounds with the highest
concentrations on 28th day were 4-p-
coumaroylquinonic acid, (-)-epicatechin,
quercetin-3-O-rutinoside, quercetin–rhamnoside,
kaempherol-3-rutinoside and quercetin-3-gucoside.
Brain lipid peroxidation (MDA) decreased (p<0.05)
on the 21st and 28th day in the PSE group.
Increase (p<0.05) in GSH concentration was
observed on the 21st and 28th and SOD activity on
the 28th day. Catalase activity was unchanged. It
could be hypothesized that highest PPH
concentration-ratios, caused reduction of lipid
peroxidation by radical scavenging and
simultaneous induction of glutathione and
SOD pathways.
Conclusions: Screened compounds could be
candidates for examining or creation of brain
targeted “neuro-nutriceuticals” polyphenol
mixtures.

POVEZANOST RADA