Naslov
The activity of garlic and ginger extracts to the epithelial damage caused by sodium taurocholate in a cell culture model of ulcer disease

Autori
Kuna, Lucija

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Medicinski fakultet Osijek

Mjesto
Osijek

Datum
10.02

Godina
2022

Stranica
112

Mentor
Smolić, Robert

Neposredni voditelj
Wu, Catherine H

Ključne riječi
peptic ulcer disease, sodium taurocholate, garlic extracts, lansoprazole, AGS cell line

Sažetak
Objectives: PUD is a chronic disease affecting up to 10% of the world's population. A cellular model of PUD can be established in AGS by NaT. The aim of the study was to explore effects of GE pretreatment and LPZ addition in the cell culture model of PUD by examining oxidative stress, F- actin distribution and determine morphological changes in cell membrane structure. Study design: Cells were plated at a density of 4x105 cells/mL in 6-well plates and were grown for 24 hours. Cells were pretreated with LPZ and GE for 24, 48 and 72 hours and subsequently exposed to NaT for 1 h. Material and methods: The AGS cell line was used as a model of PUD. The establishment of the NaT model was determined by the MTT test. Evaluation was done by determination of mitotic potential of the cells by by staining against (PCNA) ; GSH and PGE2 concentrations by ELISA ; AGS proliferation by cell counting ; expression of SOD, ABCG2, NFKB2 and TRX1 by RT PCR ; F-actin cytoskeleton visualization by semi-quantification of Rhodamine Phalloidin stain ; morphological changes in cell membrane structure by using CL and PH specific stains. Results: Our results showed significant reduction of cell damage after NaT incubation when the AGS cells were pretreated with LPZ (p<0.001) and increasing concentrations of GE (p<0.001). Pretreatment with different concentrations of GE increased PGE2 and suppressed depletion of GSH (p<0.001). Positive correlation of SOD, NFKB2 (p<0.01) and TRX 1 (p<0.001) with LPZ and GE pretreatment were seen, while ABCG2 expression was not changed. Pretreatment of the cells with GE reverses changes in the cell membrane composition and cytoskeletal protein levels induced by NaT exposure (p<0.001). Conclusion: GE pretreatment had gastroprotective effect in the cell model of PUD. Further experiments are needed to fully elucidate the mechanism of the protective role of GE in PUD.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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