Naslov
Quantitative in-vivo magnetic resonance imaging of moderate perinatal hypoxia-related changes in a rat brain
(Quantitative in-vivo magnetic resonance imaging of moderate perinatal hypoxia-related changes in a rat brain)

Autori
Drlje, Matea ; Trnski, Sara ; Štajduhar, Andrija ; Nikolić, Barbara ; Bobić- Rasonja, Mihaela ; Hranilović, Dubravka ; Škokić, Siniša ; Jovanov Milošević, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
022 Neuroscience Meeting Planner / - , 2022

Skup
Neuroscience 2022

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 12.11.2022. - 16.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MRI ; development ; hypoxia

Sažetak
AimThe in-vivo MRI study of juvenile rats aims to detect neuroradiological structural and volumetric changes consequently to a moderate perinatal hypoxic brain lesion. MethodsSixteen male Wistar Han (RccHan: WIST) rats were subjected to moderate hypoxia (8% O2, 92% N2/2h, ( 8 treated and 8 control) on the first postnatal day (P1). On P15, MRI T2-weighted scans were obtained in-vivo, and brain volume was quantified after manual delineation of regions of interest, using ITK-SNAP and custom scripts in Python 3.8. In addition, 16 post-hypoxia (8 males, 8 females) rats were sacrificed on P50, brains were instantly isolated, and immediately weighed on a digital scale. Due to the sex-related difference in size, brain mass was compared separately in males and females by student t-test. ResultsThe rats subjected to hypoxia show an indicative increase in olfactory bulb volume fraction (VF) median from the total brain volume (TBV ; 4.36+- 0.78%) and 4.55+- 0.37%, p=0, 0734). We found no difference between groups in the TBV or ventricular system VF medians. Post-hypoxic adult animals displayed a slight but significant increase in the brain mass in both males(1, 81+- 0.06 g and 1.72 +- 0.03 g, respectively, p= 0.0032) and females (1.67+- 0.05 g and 1.54+- 0.15 g, respectively, p=0.0448), in comparison to control rats. ConclusionFurther investigation is needed to understand the underlying mechanisms of demonstrated brain volume and mass changes after moderate perinatal hypoxia.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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