Naslov
Sodium/potassium ATPase activity and protein expression exhibit an age-dependent profile in mice with altered ganglioside composition
(Sodium/potassium ATPase activity and protein expression exhibit an age-dependent profile in mice with altered ganglioside composition)

Autori
Puljko, Borna ; Stojanović, Mario ; Ilić, Katarina ; Maček Hrvat, Nikolina ; Heffer, Marija ; Balog, Marta ; Mlinac Jerković, Kristina ; Kalanj Bognar, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
FEBS - IUBMB - ENABLE 1st International Molecular Biosciences PhD and Postdoc Conference

Mjesto i datum
Sevilla, Španjolska, 16.11.2022. - 18.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sodium/potassium ATPase ; gangliosides ; lipid rafts

Sažetak
Gangliosides, membrane glycosphingolipids particularly abundant in nervous tissue, have an important role in signaling events affecting neural development and pathogenesis of various neurological and neurodegenerative diseases. Gangliosides undergo extensive interactions with membrane proteins affecting their function. One amongst them is Na+/K+-ATPase (NKA), a membrane ion transporter with many vital roles including the stabilization of the resting membrane potential and regulation of ion homeostasis. The aim of this study was to investigate the effect of gangliosides on NKA activity and protein expression using the ST8sia1 null mouse model with impaired ganglioside synthesis. Wild type (WT) and null mice littermates of different ages (1 and 6 months old) were sacrificed and their brains neuroanatomically dissected. NKA activity was determined in cortical homogenates spectrophotometrically. Protein expression was analyzed by Western blotting. Results revealed a difference in both the activity and protein expression between the different age groups, with the activity being unchanged in infant mice and statistically lower in adult null mice compared to their WTs. Protein expression was found to exhibit a diametrically opposite profile, with NKA expression being higher in infants, yet statistically lower in adult null mice compared to WTs. These results clearly demonstrate the imminent impact of gangliosides on NKA and are in compliance with the fact that ganglioside composition changes during brain development and aging. A change in NKA function due to altered ganglioside composition may contribute to the development of neurodegenerative or neurological diseases by causing changes in ion homeostasis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA