Naslov
Resistance of yeast cells to the anti-cancer drug 5-fluorouracil is leveraged by regulating levels of RNA exosome complex subunits and cofactors

Autori
Novačić, Ana ; Oskomić, Marina ; Žunar, Bojan ; Primig, Michael ; Stuparević, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of FEBS - IUBMB - ENABLE 1st International Molecular Biosciences PhD and Postdoc Conference: The perfect tandem: How technology expands the frontiers of biomedicine / - , 2022, 152-152

Skup
FEBS - IUBMB - ENABLE 1st International Molecular Biosciences PhD and Postdoc Conference: The perfect tandem: How technology expands the frontiers of biomedicine

Mjesto i datum
Sevilla, Španjolska, 16.-18.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
5-fluorouracil ; RNA exosome ; yeast ; cancer resistance

Sažetak
The RNA exosome is a conserved complex that catalyzes 3’→5’ degradation and processing of RNA molecules in the nucleus and cytosol of eukaryotic cells. This complex was shown to be involved in the cellular resistance to the anti-cancer drug 5-fluorouracil (5-FU) in yeast Saccharomyces cerevisiae and human cells. 5-FU is an anti-metabolite that is incorporated into DNA and RNA molecules and interferes with various cellular processes, which is why it is widely used for the treatment of colon and breast cancer. However, the development of resistance to 5-FU can be a major obstacle to 5-FU-based therapies, and the molecular mechanisms underlying such resistance remain poorly understood. In this work, we show that expression of the RNA exosome catalytic subunit Rrp6 is regulated by 5-FU treatment in two evolutionarily distant yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Furthermore, we found that artificially up- or down-regulating the levels of RNA exosome complex subunits and cofactors through overexpression or gene deletion influences the resistance of yeast cells to 5-FU. Given the high degree of conservation of the RNA exosome complex and its cofactors, these results might contribute to elucidation of general molecular mechanisms which underlie 5-FU resistance.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA