Naslov
The stereological analysis of the human subthalamic nucleus

Autori
Bokulić, Ema ; Medenica, Tila ; Knezović, Vinka ; Štajduhar, Andrija ; Sedmak, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Virtual FENS regional meeting 2021 : book of abstracts / - Krakov, 2021, 89-89

Skup
FENS regional meeting (FRM 2021)

Mjesto i datum
Online, 25.08.2021. - 27.08.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
human brain ; immunohistochemistry ; stereology ; subthalamic nucleus

Sažetak
The subthalamic nucleus (STN) is a small, lens- shaped structure providing the only glutamatergic input to the basal ganglia circuits. Like other basal ganglia structures, the nucleus is conventionally divided in three functional parts: motor, associative, and limbic territory. This subdivision is particularly useful for planning neurosurgical procedures like deep brain stimulation in which electrodes are implanted in the motor portion of the nucleus. However, the number of STN subdivisions reported in literature varies from 0 to 4 and the intricacies of its cellular composition are not well understood [1, 2] . In order to further explore STN neuronal populations, we performed immunohistochemical analysis of several well-known neuronal markers (NeuN, parvalbumin, calretinin, calbindin, nNOS) and transcription factors implicated in specifying neuronal phenotype (FOXP2, PAX6, NKX2.1) on post mortem adult human STN [3]. Using Stereo Investigator software, we estimated neuronal density, counted the number of immunoreactive neurons for each marker, and described their spatial distribution. The coordinates of neurons and contours of each STN were extracted to enable the final visualization of data using custom Python scripts. Generally, the analysed markers can be divided into two categories: one with density greater than 3000 neurons/mm3 (nNOS, PAX6, Nissl, FOXP2) and second with density around 2.200 neurons/mm3 (NeuN, NKX2.1, parvalbumin, calretinin). We didn’t observe clearly delineated zones of expression for each marker, but we detected a gradual increase of neuronal density from dorsolateral to ventromedial tip of the nucleus.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti

POVEZANOST RADA