Naslov
MALDI TOF mass spectrometry for identification aerobic mesophilic bacteria in raw and preserved milk

Autori
Kazazić, Snježana ; Lovrić, Nina ; Darrer, Monica ; Mikulec, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th International Congress on Food Safety and Quality “One Health”, Archives of Industrial Hygiene and Toxicology, 73, Suppl. 1 / Kolarić, Branko ; Krivohlavek, Adela ; Bošnir, Jasna ; Šikić, Sandra - Zagreb : Institute for Medical Research and Occupational Health, Zagreb, Republic of Croatia, 2022, 43-43

Skup
4 međunarodni skup o sigurnosti hrane i kvaliteti hrane: Jedno zdravlje = 4th International Congress on Food Safety and Quality “One Health”

Mjesto i datum
Dubrovnik, Hrvatska, 09.11.2022. - 12.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
azidiol ; protočna citometrija ; higijenska kvaliteta
(azidiol ; flow cytometry ; hygienic quality)

Sažetak
The number of aerobic mesophilic bacteria in milk is one of the indicators of the hygienic quality of milk. A quantity greater than prescribed indicates inadequate hygiene during production and processing and contamination represents a potential health hazard and negatively affects the quality of milk and milk products. The aim of this work was to determine aerobic mesophilic bacteria and their number in raw unpreserved milk and milk preserved with azidiol. In 40 samples, the total number of aerobic mesophilic bacteria was determined using the flow cytometry method and the classic method of counting colonies on a nutrient medium according to the international standard HRN EN ISO 4833-1:2013. The results showed a decreasing trend regarding the number of grown colonies in milk preserved with azidiol. The number of samples was too small to conclude a generally negative influence of azidiol on the total number of aerobic mesophilic bacteria in milk. With matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, it is possible to quickly and easily identify bacteria at the species and genus level. This technique successfully identified 298 bacterial colonies in raw milk samples and 243 colonies in preserved milk samples. Twenty-nine genera and 45 bacterial species were identified in the raw milk samples, and 25 genera and 36 bacterial species were identified in the preserved samples. This way, a more detailed insight into the hygienic quality of milk and influence of preservatives on the presence of certain species before and after being preserved with azidiol was obtained.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA