Naslov
IL6R Polymorphism rs2228145 and GP130 Polymorphism rs3729960 do not Contribute to Microsatellite Instability in Sporadic Colorectal Cancer

Autori
Salar, Anamarija ; Čačev, Tamara ; Kapitanović, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
“HDIR-6: Targeting Cancer” The 6th Meeting of the Croatian Association for Cancer Research with International Participation : Book of Abstracts / Ozretić, Petar - Zagreb : Hrvatsko društvo za istraživanje raka (HDIR), 2022, 48-48

ISBN
978-953-48672-1-1

Skup
6th Meeting of the Croatian Association for Cancer Research with International Participation: Targeting Cancer (HDIR-6)

Mjesto i datum
Zagreb, Hrvatska, 10.11.2022. - 12.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
IL6 ; IL6R ; gp130 ; sporadic colorectal cancer

Sažetak
Chronic inflammation over time contributes to the accumulation of genetic and epigenetic alterations leading to malignant transformation. Elevated microsatellite alterations at selected tetranucleotide repeats (EMAST) is a biomarker of a specific kind of microsatellite instability caused by hMSH3 dysfunction due to chronic inflammation. Interleukin 6 (IL6) represents one of the mediators of chronic inflammation whose high serum levels contribute to the formation and progression of tumors. Signal transmission via IL6 can be classical and trans, the latter being associated with pro-inflammatory and pro- tumorigenic effects. Since IL6 receptor is not present on colon cells they can only undergo trans-signaling. In addition, soluble form of IL6 coreceptor gp130 acts as a selective antagonist of the IL6 trans-signaling pathway. Thus the response of colon cells to IL-6 is regulated by the amount of IL6, the presence of the soluble form of IL6R, and the presence of the soluble and membrane form of gp130 coreceptor. The aim of this study was to examine the distribution of two functional polymorphisms one in the IL6 receptor (rs2228145 ; 48892 A>C) and another in the gp130 coreceptor (rs3729960 ; 148 G>C) in tumors classified according to the different types of microsatellite instability. In this study, we have examined the IL-6R rs2228145 and gp130 rs3729960 polymorphisms in 190 sporadic colorectal tumors. The PCR-RFLP followed by either agarose gel or polyacrylamide electrophoresis was used for SNP genotyping and the data were examined according to the presence or absence of a specific type of microsatellite instability. Homozygous carriers of functional variants of rs2228145 and rs3729960 which have previously been associated with regulatory influence on IL6R and gp130 were not associated with either MSI or EMAST type of microsatellite instability. We can conclude that since neither rs2228145 variant allele C nor rs3729960 variant allele C were more common in tumors with either MSI or EMAST type of microsatellite instability they are not contributing to these two types of genome instability.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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