Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1230188

GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY


Ivana Rubić, Alan Kovačević, Tomislav Mašek, Josipa Kuleš, Renata Barić Rafaj, Vladimir Mrljak
GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY // 47th World Small Animal Veterinary Association World Congress and the 28th FIAVAC Congress / Frederic Gaschen, Mary Marcondes (ur.).
Lima: WSAVA, 2022. P52 / #447, 1 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1230188 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY

Autori
Ivana Rubić, Alan Kovačević, Tomislav Mašek, Josipa Kuleš, Renata Barić Rafaj, Vladimir Mrljak

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
47th World Small Animal Veterinary Association World Congress and the 28th FIAVAC Congress

Mjesto i datum
Lima, Peru, 29.10.2022. - 31.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
GC-MS metabolomics, dogs, IDCM

Sažetak
Introduction: The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. iDCM is a primary myocardial disease characterized by systolic dysfunction and eccentric hypertrophy primarily of the left or both ventricles at the same time. The prognosis of the disease is generally unfavorable. Objectives: The goal was to investigate novel metabolites and possible changes in the plasma metabolome of dogs with iDCM and healthy dogs using gas chromatography-mass spectrometry (GC- MS). Methods: Plasma of 20 dogs with iDCM were analysed in the retrospective study. Metabolite extraction were performed using the GC-MS metabolomics sample preparation protocol. In short, the plasma samples were extracted with extraction solvent, incubated, centrifuged and evaporated to dryness using a speedvac concentrator (Thermo Fisher Scientific). Instrumental analysis was carried out on a Shimadzu single quadrupole GCMS-QP2010 gas chromatograph-mass spectrometer (Shimadzu). Metabolites were identified using a commercially available GC-MS Metabolite Mass Spectral Database (Shimadzu). Results: GC-MS metabolomics analysis identified 25 metabolites in plasma samples from 20 dogs. The data processing determined that 10 of them were amino acids, 8 were organic acids, 3 were carbohydrates, 3 were fatty acids, and sugar alcohol. Statistical analysis showed that lactic acid, citric acid and alanine were more abundant in dogs with iDCM compare to the healthy dogs. Conclusions: Investigation of plasma samples from dogs with iDCM distinguishes significantly from healthy dogs. The GC-MS metabolomics revealed potential novel metabolites in the canine plasma metabolome.

Izvorni jezik
Engleski



POVEZANOST RADA



Citiraj ovu publikaciju:

Ivana Rubić, Alan Kovačević, Tomislav Mašek, Josipa Kuleš, Renata Barić Rafaj, Vladimir Mrljak
GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY // 47th World Small Animal Veterinary Association World Congress and the 28th FIAVAC Congress / Frederic Gaschen, Mary Marcondes (ur.).
Lima: WSAVA, 2022. P52 / #447, 1 (poster, međunarodna recenzija, sažetak, znanstveni)
Ivana Rubić, Alan Kovačević, Tomislav Mašek, Josipa Kuleš, Renata Barić Rafaj, Vladimir Mrljak (2022) GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY. U: Frederic Gaschen, M. (ur.)47th World Small Animal Veterinary Association World Congress and the 28th FIAVAC Congress.
@article{article, editor = {Frederic Gaschen, M.}, year = {2022}, pages = {1}, chapter = {P52 / \#447}, keywords = {GC-MS metabolomics, dogs, IDCM}, title = {GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY}, keyword = {GC-MS metabolomics, dogs, IDCM}, publisher = {WSAVA}, publisherplace = {Lima, Peru}, chapternumber = {P52 / \#447} }
@article{article, editor = {Frederic Gaschen, M.}, year = {2022}, pages = {1}, chapter = {P52 / \#447}, keywords = {GC-MS metabolomics, dogs, IDCM}, title = {GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY}, keyword = {GC-MS metabolomics, dogs, IDCM}, publisher = {WSAVA}, publisherplace = {Lima, Peru}, chapternumber = {P52 / \#447} }




Contrast
Increase Font
Decrease Font
Dyslexic Font