Pregled bibliografske jedinice broj: 1230188
GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY
GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY // 47th World Small Animal Veterinary Association World Congress and the 28th FIAVAC Congress / Frederic Gaschen, Mary Marcondes (ur.).
Lima: WSAVA, 2022. P52 / #447, 1 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1230188 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES
FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY
Autori
Ivana Rubić, Alan Kovačević, Tomislav Mašek, Josipa Kuleš, Renata Barić Rafaj, Vladimir Mrljak
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
47th World Small Animal Veterinary Association World Congress and the 28th FIAVAC Congress
Mjesto i datum
Lima, Peru, 29.10.2022. - 31.10.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
GC-MS metabolomics, dogs, IDCM
Sažetak
Introduction: The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. iDCM is a primary myocardial disease characterized by systolic dysfunction and eccentric hypertrophy primarily of the left or both ventricles at the same time. The prognosis of the disease is generally unfavorable. Objectives: The goal was to investigate novel metabolites and possible changes in the plasma metabolome of dogs with iDCM and healthy dogs using gas chromatography-mass spectrometry (GC- MS). Methods: Plasma of 20 dogs with iDCM were analysed in the retrospective study. Metabolite extraction were performed using the GC-MS metabolomics sample preparation protocol. In short, the plasma samples were extracted with extraction solvent, incubated, centrifuged and evaporated to dryness using a speedvac concentrator (Thermo Fisher Scientific). Instrumental analysis was carried out on a Shimadzu single quadrupole GCMS-QP2010 gas chromatograph-mass spectrometer (Shimadzu). Metabolites were identified using a commercially available GC-MS Metabolite Mass Spectral Database (Shimadzu). Results: GC-MS metabolomics analysis identified 25 metabolites in plasma samples from 20 dogs. The data processing determined that 10 of them were amino acids, 8 were organic acids, 3 were carbohydrates, 3 were fatty acids, and sugar alcohol. Statistical analysis showed that lactic acid, citric acid and alanine were more abundant in dogs with iDCM compare to the healthy dogs. Conclusions: Investigation of plasma samples from dogs with iDCM distinguishes significantly from healthy dogs. The GC-MS metabolomics revealed potential novel metabolites in the canine plasma metabolome.
Izvorni jezik
Engleski
POVEZANOST RADA
Profili:
Alan Kovačević
(autor)
Vladimir Mrljak
(autor)
Renata Barić-Rafaj
(autor)
Ivana Rubić
(autor)
Tomislav Mašek
(autor)
Josipa Kuleš
(autor)