Naslov
Protein expression profile of Na+/K+-ATPase in specific brain regions of mice with altered ganglioside composition

Autori
Puljko, Borna ; Stojanović, Mario ; Ilić, Katarina ; Maček Hrvat, Nikolina ; Heffer, Marija ; Mlinac Jerković, Kristina ; Kalanj Bognar, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB22 From Science to Knowledge Book of Abstracts / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb, 2022, 1-1

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gangliosides, sodium/potassium ATPase

Sažetak
Gangliosides are membrane glycosphingolipids particularly abundant in mammalian nervous tissue. Being harboured in the outer leaflet of the plasma membrane, they are implicated in various physiological processes including brain development, aging and signal transduction pathways by modulating the function and structure of specific membrane proteins. Our previous studies revealed that Na⁺/K⁺-ATPase (NKA), a membrane ion transporter, appears to be one of the proteins affected by ganglioside composition. NKA dysfunction leads to a disbalance in ion homeostasis in nervous tissue reported in numerous neurodegenerative and neurological diseases. Although the effect of gangliosides on NKA had been observed, detailed clarification of their interrelations still awaits elucidation. Hence, we investigated NKA protein expression and localization in brain tissue derived from the St8sia1 null mice - a transgenic mouse model with impaired synthesis of gangliosides, characterized by lack of the b- and c- series and overexpression of the a-series gangliosides. Protein expression of NKA was determined in cortical and cerebellar tissue homogenates from the null and wild-type (WT) mice by Western blotting using the NKA-specific antibody. Immunohistochemical staining was performed on frozen brain tissue slices from the null and WT mice using the same NKA- specific antibody. Results revealed a statistically lower amount of NKA expressed in the cortices of null mice compared to WTs, with no disparity in the cerebella. Immunohistochemical staining revealed the lower intensity of NKA staining in the cortical tissue and cerebellar molecular and granular layers of null mice compared to WT mice. These results clearly demonstrate and strengthen our previously reported findings that NKA is affected by ganglioside environment in a way that gangliosides provide the proper lipid milieu needed for optimal pump functioning and ion homeostasis upkeep. Considering the vital and ubiquitous role of NKA in maintaining resting potential and regulating cellular volume, the paramount importance of ganglioside surroundings on NKA is even further highlighted and should be considered when conducting studies regarding NKA function.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA