Naslov
Isolation and characterization of exosomes isolated from tumor cells

Autori
Vasiljević, Tea ; Matijević Glavan, Tanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts - Science and Us: 1st Biomedicine and Health PhD Students Congress with International Participation / - Rijeka, 2022, 78-78

Skup
“Science and Us” The 1st Biomedicine and Health PHD Students Congress

Mjesto i datum
Rijeka, Croatia, 19.05.2022. - 20.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
exosomes ; head and neck squamous cell carcinoma ; tumor microenvironment

Sažetak
Exosomes are extracellular vesicles detectable in biological fluids that play a significant role in communication between cells. Exosomes vary in size from 30 to 150 nm. They accumulate in the tumor microenvironment since tumor cells are highly active in producing exosomes. Exosomes may carry damageassociated molecular patterns (DAMPs), consequently, their levels rise during pathological changes and decrease during recovery. Tumor-derived exosomes are proved to be increased in head and neck squamous cell carcinoma (HNSCC) patient’s plasma. Hence, exosomes can be used as a non-invasive biomarker. Cell lines used were pharyngeal cancer cell lines Detroit 562 and FaDu ; and laryngeal cancer cell line SQ20B. Exosomes were isolated using 101Bio PureExo® Exosome Isolation kit from cell culture media. Isolated exosomes were characterized using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and western blot for exosome markers. We have determined that the exosomes were successfully isolated from the tumor cell lines media. NTA determined the size of exosomes to be as expected at around 30 to 200 nm. TEM detected visible abundance of exosomes at the size 40 to 220 nm. SQ20B exosomes were detected as significantly smaller compared to exosomes from Detroit 562 and FaDu cell lines. Western blot analysis showed the presence of CD63 exosome marker. Calnexin was not detected which indicates that the exosome extract is not contaminated with other cellular vesicles. The goal was to apply the best exosome isolation method to obtain pure and high-quality exosome yield with expected size and shape of the vesicles. After a detailed review of the literature, exosome isolation kit was selected based on research and technical data. The experiments have shown that this isolation kit is indeed applicable to our research purpose. Exosomes were successfully isolated as demonstrated by NTA, TEM and western blot. In our future research we will study how tumor-derived exosomes could affect tumor progression in HNSCC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA