Pregled bibliografske jedinice broj: 1229700
(Un)coupling the factors contributing to the interfacial activation of Streptomyces rimosus lipase: computational and spectrophotometric study
(Un)coupling the factors contributing to the interfacial activation of Streptomyces rimosus lipase: computational and spectrophotometric study // Journal of dispersion science and technology (2023) doi:10.1080/01932691.2022.2145304 (znanstveni, prihvaćen)
CROSBI ID: 1229700 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
(Un)coupling the factors contributing to the
interfacial activation of Streptomyces rimosus
lipase: computational and spectrophotometric study
Autori
Ćehić, Mirsada ; Brkljača, Zlatko ; Filić, Želimira ; Crnolatac, Ivo ; Vujaklija, Dušica ; Bakarić, Danijela
Vrsta, podvrsta
Radovi u časopisima,
znanstveni
Izvornik
Journal of dispersion science and technology (2023)
Status rada
Prihvaćen
Ključne riječi
p-nitrophenylpalmitate (p-NPP) ; Streptomyces rimosus lipase (SrLip) ; Interfacial activation (IA) ; Gum Arabic ; Sodium deoxycholate ; 1, 2-Dioleyl-sn-glycero-3-phosphocholine (DOPC) liposomes
Sažetak
Streptomyces rimosus lipases (SrLip) present an enzyme class that catalyses the hydrolysis of triacylglycerols producing fatty acids and glycerols. The abrupt increase in catalytic activity of SrLip when found at water-oil interface is assigned as interfacial activation (IA). Since the latter is a complex function of interface charge, chemical composition, as well as substrate orientation and concentration, the role of individual factors and their combinations that trigger IA remains rather elusive. The aim of this work is to unravel the contribution of particular factor in IA by examining SrLip activity towards p-nitrophenylpalmitate (p-NPP) as a substrate incorporated in three chemically different self- assembled aggregates: gum Arabic, sodium deoxycholate and liposomes made of 1, 2-dioleyl- sn- glycero-3-phosphocholine (DOPC) lipids, and at two different pH values (pH = 6.8 and pH = 10.0). Spectrophotometric (UV/Vis) study revealed that SrLip catalyses p-NPP hydrolysis when the substrate is embedded in sodium deoxycholate at both pH values, suggesting that the coupling of favorable interface charge and substrate orientation drives the interfacial activation. Moreover, especially in alkaline conditions (pH = 10.0), hydrolysis of the ester bond of the substrate incorporated in DOPC bilayers occurs when appropriate orientation, concentration and arrangement is accomplished. All experiments were conducted in parallel on an inactive mutant SrLipS10/A as a reference. The molecular-level details of particular interface and accompanied substrates examined by all-atom molecular dynamics simulations revealed that availability of p-NPP at these three interfaces decreases in the following order: sodium-deoxycholate > DOPC > model gum Arabic.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
Projekti:
HRZZ-UIP-2020-02-7669 - Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima (DEMYMOLSCALE) (Bakarić, Danijela, HRZZ - 2020-02) ( CroRIS)
HRZZ-IP-2018-01-1754 - Identifikacija interaktoma paralognih proteina SSB u višestaničnom prokariotu, Streptomyces coelicolor (id_ROBUST) (Vujaklija, Dušica, HRZZ - 2018-01) ( CroRIS)
HRZZ-IP-2018-01-5475 - Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina (BioMultiChromoProbes) (Piantanida, Ivo, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Profili:
Ivo Crnolatac
(autor)
Zlatko Brkljača
(autor)
Želimira Filić
(autor)
Danijela Bakarić
(autor)
Dušica Vujaklija
(autor)
Mirsada Ćehić
(autor)