Naslov
Monitoring Air Pollution and The Health-Related Biomarkers: Lessons from HUMNap

Autori
Matković, Katarina ; Jurič, Andreja ; Jakovljević, Ivana ; Davila, Silvije ; Milić, Mirta ; Kašuba, Vilena ; Pehnec, Gordana ; Brčić Karačonji, Irena ; Gajski, Goran ; Gerić, Marko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book of the 11th International Congress of the Turkish Society of Toxicology / - , 2022, 9-9

Skup
11th International Congress of the Turkish Society of Toxicology

Mjesto i datum
Kemer, Antalija, Hrvatska, 2.-5.11.2022

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
air pollution ; BTEX ; DNA damage ; biomonitoring

Sažetak
Exposure to outdoor air pollution is related to severe health effects, especially in urban areas, due to higher concentrations of pollutants caused by urban agglomeration, traffic, and industrial activities. Additionally, the emission of BTEX (benzene, toluene, ethylbenzene, and xylene) compounds becomes higher, mainly due to intensive industrialization and urbanization. Hence, we aimed to investigate possible effects of air pollution and BTEX exposure on DNA damage detected by the alkaline comet assay and micronucleus assay on human buccal and peripheral blood cells. The first part of the study was conducted during the colder period of the year 2021 and the follow up part during the warmer period of the year 2022. It involved 60 healthy volunteers aged 18-55 years with BMI<30 kg/m2 living in Zagreb (Croatia). To evaluate potential impact of air pollution on comet and micronucleus assay results, association was made using different time windows of measured air pollution parameters prior to sampling, while BTEX were analysed in whole blood using headspace solid-phase micro extraction (SPME) followed by gas chromatography-mass spectrometry (GC-MS). All measured outdoor air pollution parameters were in agreement with previously reported values, which were bellow regulatory limit, except for PM10 particle fraction (particles with aerodynamic diameter less than 10 μm) and benzo[a]pyrene bound to PM10, that exceeded regulatory limit levels. Statistical analysis was used to investigate correlation between the measured biomarkers of exposure and DNA damage along with their association to different sampling periods, using Spearman’s correlation and Linear regression modelling. Future research will include same study design for populations living in other Croatian cities, where we expect exposure to different set of air pollutants.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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