Naslov
Development and validation of HPLC method for the determination of n-nitrosamines in water

Autori
Babić, Sandra ; Golub, Valentino ; Morović, Silvia ; Košutić, Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
IMEKO TC11 & TC24 Joint Hybrid Conference

Mjesto i datum
Cavtat, Hrvatska, 17-19.10.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HPLC, N-nitrosamines, water, validation

Sažetak
The presence of N-nitrosamines in water is considered an emerging issue because they are highly carcinogenic and mutagenic to humans at trace levels. They can enter drinking water supplies mainly as a result of disinfection processes by the reaction between disinfectant (chloramine, chlorine and ozone) and the nitrogen- containing organic matter. Furthermore, nitrosamines are formed during some industrial processes such as cosmetics and food processing, leather tanning, rubber, pesticides and dyes manufacturing which can lead to their appearance in wastewater effluents. This work reports the development and validation of an analytical method for the quantitative determination of five N-nitrosamines namely N- nitrosomorpholine (NMOR), N-nitrosopirrolidine (NPYR), N-nitrosodiethylamine (NDEA), N- nitrosodipropylamine (NDPA), and N- nitrosodibutylamine (NDBA). The method is based on high performance liquid chromatography (HPLC) coupled to photo diode array (PDA) detector using Kinetex C18 column (150 mm × 4.6 mm, particle size 5 m, Phenomenex). Chromatographic separation was optimized regarding mobile phase composition and elution mode (isocratic and gradient). Prior to HPLC analysis, the off-line SPE preconcentration step was optimized to achieve low quantification limits. A coconut charcoal sorbent provided the best results in the preconcentration of 500 mL water samples. The optimized SPE-HPLC-PDA method was validated using tap water samples. A satisfactory linearity was obtained with R2 > 0.999 and limit of quantification at ngL-1 level. Validated method has been applied for the determination of N-nitrosamines removal efficiencies by reverse osmosis (RO) membrane treatment.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA