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Pregled bibliografske jedinice broj: 1229195

Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli


Barbarić, Lea; Tomašić-Paić, Ana; Matić, Sara; Matovina, Mihaela
Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli // Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana (ur.).
Zagreb: Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2022. str. 65-65 (poster, podatak o recenziji nije dostupan, sažetak, znanstveni)


CROSBI ID: 1229195 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli

Autori
Barbarić, Lea ; Tomašić-Paić, Ana ; Matić, Sara ; Matovina, Mihaela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2022, 65-65

Skup
HDBMB22: From Science to Knowledge

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
SH2D3C, DPP3, protein expression

Sažetak
Protein-protein interactions are crucial for many cellular processes and their research allows us to understand these processes from a biochemical point of view. Since changes in the regulation of signaling pathways in the cell often lead to the development of various diseases, studying protein- protein interactions can help us better understand their origin, development and impact on human health. SH2D3C acts as an adapter protein in signaling pathways involved in cell adhesion and migration, tissue organization, and regulation of the immune response. The protein contains a Ras GEF-like C-terminal domain that has no significant GEF activity, but may interact with other proteins. Analysis of the cellular proteome by SILAC-MS revealed a potential interaction of SH2D3C with dipeptidyl peptidase III (DPP III) involved in the regulation of oxidative stress by competitively binding to the KEAP1 protein which is a key participant in the Nrf2/KEAP1 signaling pathway. This would mean that their interaction represents a possible link between the Nrf2/KEAP1 signaling pathway and cell migration regulation. To confirm the interaction by other methods such as micro-scale thermophoresis (MST), the C- terminal domain of SH2D3C was overexpressed and purified in E. coli with two different tags, GST and MBP.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
--IP-2020-02-6743 - Interakcija dipeptidil peptidaze III s proteinom SH2 domain-containing protein 3C – moguća veza između odgovora na oksidativni stres i stanične migracije (OxMiLink) (Matovina, Mihaela) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Mihaela Matovina (autor)

Avatar Url Ana Tomašić Paić (autor)

Avatar Url Lea Barbarić (autor)

Avatar Url Sara Matić (autor)

Poveznice na cjeloviti tekst rada:

congress2022.hdbmb.hr

Citiraj ovu publikaciju:

Barbarić, Lea; Tomašić-Paić, Ana; Matić, Sara; Matovina, Mihaela
Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli // Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana (ur.).
Zagreb: Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2022. str. 65-65 (poster, podatak o recenziji nije dostupan, sažetak, znanstveni)
Barbarić, L., Tomašić-Paić, A., Matić, S. & Matovina, M. (2022) Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli. U: Dulić, M., Sinčić, N. & Vrhovac Madunić, I. (ur.)Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge.
@article{article, author = {Barbari\'{c}, Lea and Toma\v{s}i\'{c}-Pai\'{c}, Ana and Mati\'{c}, Sara and Matovina, Mihaela}, year = {2022}, pages = {65-65}, keywords = {SH2D3C, DPP3, protein expression}, title = {Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli}, keyword = {SH2D3C, DPP3, protein expression}, publisher = {Hrvatsko dru\v{s}tvo za biokemiju i molekularnu biologiju (HDBMB)}, publisherplace = {Brela, Hrvatska} }
@article{article, author = {Barbari\'{c}, Lea and Toma\v{s}i\'{c}-Pai\'{c}, Ana and Mati\'{c}, Sara and Matovina, Mihaela}, year = {2022}, pages = {65-65}, keywords = {SH2D3C, DPP3, protein expression}, title = {Overexpression and purification of the c-terminal domain of SH2D3C in escherichia coli}, keyword = {SH2D3C, DPP3, protein expression}, publisher = {Hrvatsko dru\v{s}tvo za biokemiju i molekularnu biologiju (HDBMB)}, publisherplace = {Brela, Hrvatska} }




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