Naslov
Epigenetic, genetic, and expression analysis of brain-derived neurotrophic factor in Alzheimer's disease

Autori
Tudor, Lucija ; Nikolac Perković, Matea ; Babić Leko, Mirjana ; Videtič Paska, Alja ; Kouter, Katarina ; Miloš, Tina ; Nedić Erjavec, Gordana ; Vuić, Barbara ; Konjevod, Marcela ; Šimić, Goran ; Borovečki, Fran ; Pivac, Nela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final programme and abstracts from the 10th Croatian Congress of Pharmacology and the 1st Croatian Congress of Clinical Pharmacology and Therapeutics with international participation , Pharmaca 2022 ; 52 (Suppl. 2) / Mršić-Pelčić, Jasenka ; Vitezić, Dinko ; Janković, Tamara - Zagreb, 2022, 75-75

Skup
10. hrvatski kongres farmakologije ; 1. hrvatski kongres kliničke farmakologije s međunarodnim sudjelovanjem = 10th Croatian Congress of Pharmacology ; 1st Croatian Congress of Clinical Pharmacology and Therapeutics with International Participation

Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Alzheimer's disease ; amyloid-beta1-42 ; brain-derived neurotrophic factor (BDNF) ; BDNF gene polymorphisms ; cerebrospinal fluid ; methylation status ; next generation sequencing ; plasma ; quantitative PCR

Sažetak
Introduction The most common dementia type is Alzheimer's disease (AD), characterized by progressive cognitive decline and neuronal death, which is often attributed to the accumulation of amyloid-beta (Aβ) plaques and neurofibrillary tangles in the brain. Brain-derived neurotrophic factor (BDNF) is a neurotrophin that plays an important role in neurogenesis and neuroplasticity. Therefore, our aim was to investigate the BDNF alternations in AD, at the genetic, epigenetic, and expression levels. Materials and methods The study included 254 patients with AD and mild cognitive impairment (MCI), as a comparative group. Plasma BDNF and cerebrospinal fluid (CSF) Aβ1–42 protein levels were measured with enzyme-linked immunosorbent assay. Quantitative PCR was used to determine the five BDNF gene polymorphisms and BDNF mRNA expression in peripheral white blood cells. The next-generation sequencing was used to determine the methylation status of nine amplicons covering 169 CpG sites in the BDNF gene region. The data were analyzed using GraphPad Prism v4.00 software. Results The results demonstrated increased BDNF plasma concentration in AD patients compared to MCI group, and its positive correlation with Aβ1–42 CSF levels. Tested BDNF polymorphisms were not associated with AD ; however, lower methylation levels, especially in BDNF3 and BDNF9 amplicon region and lower expression of the BDNF gene in peripheral blood were detected in AD patients, compared to MCI subjects. Conclusions These findings could help to elucidate the complex role of BDNF in AD, as well as its potential as an easily accessible peripheral biomarker and novel therapeutic strategy for AD.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Kognitivna znanost (prirodne, tehničke, biomedicina i zdravstvo, društvene i humanističke znanosti), Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA