Pregled bibliografske jedinice broj: 1228467
The STAR assay: A new sensitive, selective, and versatile for isolation of DNA-protein crosslinks
The STAR assay: A new sensitive, selective, and versatile for isolation of DNA-protein crosslinks // International Congress of the Croatian Society of Biochemistry and Molecular Biology ; From Science to Knowledge, HDBMB2022 / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana (ur.).
Zagreb: Hrvatsko Društvo za Biotehnologiju, 2022. str. 83-83 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1228467 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The STAR assay: A new sensitive, selective, and
versatile for isolation of DNA-protein crosslinks
Autori
Glumac, Mateo ; Polovic, Mirjana ; Batel, Anja ; Gelemanovic, Andrea ; Marinovic-Terzic, Ivana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
International Congress of the Croatian Society of Biochemistry and Molecular Biology ; From Science to Knowledge, HDBMB2022
/ Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2022, 83-83
Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)
Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
DPC repair ; SPRTN ; DNA repair
Sažetak
DNA-protein crosslinks (DPCs) are toxic DNA lesions created by the covalent conjugation of proteins onto DNA molecule. They can be created endogenously or exogenously by exposing DNA or surrounding proteins to physical or chemical insults or created by faulty enzymatic reactions. Because of their bulky nature and chemical changes to DNA molecule, they interfere with DNA-linked processes such as replication and transcription. With the recent discovery of proteases and nucleases that participate in the repair of such lesions, the development of an accurate biochemical assay for DPC isolation became a priority for the further mechanistic understanding of their repair. We have developed a novel assay for direct quantification of DPCs, with high sensitivity to physiologically relevant treatment conditions. The STAR assay is a sensitive, selective, and versatile method for direct quantification of DPCs and is efficient in detecting physically, chemically, and enzymatically created DNA-protein adducts. The STAR assay is the first assay to demonstrate that nuclear presence is necessary for proteins to become cross-linked to DNA molecule. Employing the STAR assay, we have demonstrated that DPC repair is a two-step process, starting with the removal of the protein part of the lesion by dedicated DNA-dependent protease SPRTN, followed by the repair of the DNA molecule itself. The STAR assay is fast and simple and is applicable for studying the formation, repair, and biological significance of DPCs.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-3097 - Uloga proteina Spartan u DNA replikaciji (SprtnRep) (Marinović Terzić, Ivana, HRZZ - 2016-06) ( CroRIS)
Ustanove:
Medicinski fakultet, Split,
Mediteranski institut za istraživanje života
Profili:
Ivana Marinović Terzić
(autor)
Mirjana Polović
(autor)
Mateo Glumac
(autor)
Anja Batel
(autor)
Andrea Gelemanović
(autor)