Pregled bibliografske jedinice broj: 1228239
Prevalence of occult hepatitis B virus infection and characterization of HBV surface antigen mutants among individuals in Primorsko-Goranska County, Croatia over a five-year period
Prevalence of occult hepatitis B virus infection and characterization of HBV surface antigen mutants among individuals in Primorsko-Goranska County, Croatia over a five-year period // 13. Hrvatski kongres klinićke mikrobiologije 10. Hrvatski kongres o infektivnim bolestima
Šibenik, Hrvatska, 2022. (poster, domaća recenzija, neobjavljeni rad, znanstveni)
CROSBI ID: 1228239 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Prevalence of occult hepatitis B virus infection
and characterization of HBV surface antigen
mutants among individuals in Primorsko-Goranska
County, Croatia over a five-year period
Autori
Bubonja-Šonje, Marina ; Mohar Vitezić, Bojana ; Peruč, Dolores ; Peračković, Martina ; Abram, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni
Skup
13. Hrvatski kongres klinićke mikrobiologije 10. Hrvatski kongres o infektivnim bolestima
Mjesto i datum
Šibenik, Hrvatska, 20-23.10.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
occult hepatitis B virus infection, HBV surface antigen mutants
Sažetak
Aim: To determine the prevalence and virological characteristics of occult hepatitis B virus (HBV) infection (OBI) in adult individuals from Primorsko-Goranska County over a five-year period. Materials and methods: Serum samples from selected anti-HBc-positive/HBsAg-negative patients (Architect, Abbott) were analyzed for the presence of HBV DNA by real-time and nested PCR. Mutational analyses of HBs-gene and polymerase gene sequences were performed using the Genetic Analyzer ABI 310 sequencer and ABI PRISM Sequencing Analysis v 5.4. software. Nucleotide sequences were aligned using MEGA software version 11. Alignment was performed using the Clustal Omega -1.2.4 program. Results: Of 24 900 sera routinely screened for HBsAg, anti-HBs and anti-HBc, 113 (0, 45%) sera were found to have an isolated anti-HBc serologic pattern. HBV DNA was detected in 12 of 113 (10, 6%) “anti-HBc only” positive sera representing 0, 048% of all routinely tested samples. Amplification of both HBV genomic segments, and sequencing results were obtained for only two samples with viremia of 50-100 IU/ml, in which subgenotype D3 were confirmed. This was expected given the extremely low viremia <6 IU/ml in the other ten HBV DNA positive samples. Mutational analyses of S-gene revealed several missense mutations. The mutations S136Y and G145R lead to a change in the polarity of aminoacids: S136Y (hydrophilic to hydrophobic) and G145R (hydrophobic to hydrophilic). Conclusion: OBI prevalence rate among the “anti- HBc only” positive population was 10, 6%. Multiple mutations of HBsAg (“S-escape” mutations) were partly responsible for its failure to be detected by a commercial serological assay (1, 77% cases). Because individuals with an isolated anti-HBc serologic pattern may have low grade chronic infection or infection with mutant strains, molecular testing is recommended on each “anti-HBc only” positive individual. The sensitivity of commercial HBsAg tests that can detect HBsAg surface mutants needs to be further improved.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
--uniri-biomed-18-215 - Proširenost i klinički značaj HBs antigenih mutanti virusa hepatitisa B (Bubonja Šonje, Marina) ( CroRIS)
--uniri-biomed-18-277 - Bakterijska patogeneza - od istraživanja do kliničke primjene (Abram, Maja) ( CroRIS)
Profili:
Marina Bubonja Šonje
(autor)
Bojana Mohar Vitezić
(autor)
Dolores Peruč
(autor)
Maja Abram
(autor)