Pregled bibliografske jedinice broj: 1228102
Fascioloides magna – the development of host-parasite interactions in Europe
Fascioloides magna – the development of host-parasite interactions in Europe // Book of Abstracts / Konjević, Dean ; Bujanić, Miljenko ; Škvorc, Nikolina (ur.).
Osijek: Veteriranrski fakultet Sveučilišta u Zagrebu, 2022. str. 32-33 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1228102 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Fascioloides magna – the development of host-parasite interactions in Europe
Autori
Konjević , Dean
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts
/ Konjević, Dean ; Bujanić, Miljenko ; Škvorc, Nikolina - Osijek : Veteriranrski fakultet Sveučilišta u Zagrebu, 2022, 32-33
ISBN
978-953-8006-39-5
Skup
10th International Deer Biology Congress
Mjesto i datum
Osijek, Hrvatska, 04.09.2022. - 09.09.2022
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Fascioloides magna, hosts, Europe, host-parasite interactions, macroscopic analysis, MHC genes
Sažetak
Context: Exactly 137 years ago, Roberto Bassi published his description of a new trematode species found in the livers of a wapiti in the Royal Park ‘La Mandria’ near Turin in Italy. Subsequently, he named this species Distomum magnum. Later it was re-named Fascioloides magna. Despite its apparent long presence in Europe, the geographical spread of F. magna is a gradual and ongoing process. Therefore, its impact on European host species can be monitored separately within different habitats. This parasite was detected for the first time in Croatia in 2002. Aims: To analyze, on a molecular and macroscopic level, the host-parasite interactions between F. magna and three different types of hosts ; definitive, aberrant and dead-end. Methods: In this study, case-controlled studies of liver from red deer, fallow deer, roe deer and wild boar were collected during the regular game management operations, based on non-probability convenient sampling. Livers were analyzed macroscopically for signs indicative of F. magna infection – traces of iron porphyrin, fibrin deposits, and liver enlargement, irregular liver surface and opaque Glisson’s capsule. Each liver was then cut into slices approx. 2 cm wide and thoroughly examined for both gross lesions and flukes on the cut section. Tissue samples were collected and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Sequencing was performed using the Illumina platform. DRB genotyping was done in the AmpliSAS web tool. The obtained sequences were compared with previously known SLA alleles using the BLAST tool (https:// blast.ncbi.nlm.nih.gov). A statistical analysis was performed in the program R, using the chi-square test and Fisher-Irwin test. Key results: Macroscopically, two potential modifications in the host-parasite interactions were observed. In the case of roe deer (an aberrant host), pseudocyst formation and the presence of a chronic infection was observed in 34 out of 227 analyzed livers. In wild boar, atypically for dead-end host, a large thin-walled pseudocyst was observed containing sexually mature flukes. Genetically, MHC DRB exon 2 allele indicative of potential association with F. magna infection were detected in both red deer and wild boar. Conclusions: Host-parasite interactions are dynamic processes in which both the host and parasite are trying to outsmart each other, and most importantly survive. Observed changes and innovations indicate potential adaptations of both species to these relatively new associations.
Izvorni jezik
Engleski
Znanstvena područja
Veterinarska medicina
POVEZANOST RADA
Projekti:
HRZZ-IP-2018-01-8963 - Interakcija nositelj-parazit: odnos tri različita tipa nositelja prema invaziji metiljem Fascioloides magna (MAGNA) (Konjević, Dean, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Veterinarski fakultet, Zagreb
Profili:
Dean Konjević (autor)