Naslov
Graphene-based in-vitro cell-culturing surfaces alter morphological properties of neonatal spiral ganglion neurons

Autori
Žarković, Jelena ; Bedalov, Ana ; Delipetar, Boris ; Marjanović, Tihomir ; Blašković, Milan ; Garaj, Slaven ; Kovačić, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Zagreb Bioelectronics Workshop and Croatian Neuroelectronics Symposium 2021

Mjesto i datum
Zagreb, Hrvatska, 4-6.11.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Spiral ganglion neurons, Graphene, Morphology

Sažetak
A new generation of neural interfaces based on novel graphene-based materials is expected to address limitations of low spatial resolution and broad specificity of neuronal stimulation in current cochlear implant devices leading to poor pitch perception and significant variability in its efficacy. Graphene and other twodimensional (2D) materials possess various properties, making them an attractive candidate for future cochlear implants. It was previously shown that graphene might support neural growth due to its unique electric properties, biocompatibility with neuronal cells, and ability to effectively interface with neuronal tissue. However, it is not known how graphene interacts with spiral ganglion neurons (SGN) morphology. In this study, three types of graphene-based substrates (single layer graphene - SLG, hydrogenated graphene - HG, and fluorinated graphene - FG) were used as a surface for in-vitro culturing of neonatal SGN extracted from rat pups. The cultures were immunocytochemically stained at eight days in vitro (8DIV), and the subsequent fluorescence images were analyzed with the custom- made machine learning-based image processing allowing automatic identification and segmentation of neurons. We compared morphological properties of neuronal cultures grown on graphene-based and single-layer h-BN surfaces with those grown on glass coverslips. We found that neonatal SGN neurons may grow on all four tested substrates with significantly altered morphological properties compared to standard glass coverslips. Analysis of the mixed effect model of different morphological parameters data revealed that HG and SLG substrates represented a more favorable environment for spiral ganglion neuron growth than those grown on control coverslips. These findings reveal substantial interaction between material properties of graphene-based substrates on in-vitro SGN cell cultures.

Izvorni jezik
Engleski

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