Naslov
Isoenzymes of peroxidase and esterase related to morphogenesis in Mammillaria gracillis Pfeiff. tissue culture

Autori
Balen, Biljana ; Krsnik-Rasol, Marijana ; Simeon-Rudolf, Vera

Izvornik
Journal of plant physiology (0176-1617) 160 (2003), 11; 1401-1406

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cactaceae; crown-gall tumour; differentiation; habituated callus; isoesterases; isoperoxidases; Mammillaria gracillis Pfeiff.

Sažetak
In vitro propagated plants of the cactus Mammillaria gracillis Pfeiff. (Cactaceae) spontaneously produced callus. The habituated callus regenerated normal and hyperhydric shoots without the addition of growth regulators. Tumours were obtained by infecting cactus explants with Agrobacterium tumefaciens ; the wild strain B6S3 (tumour line TW) or with the rooty mutant GV3101 (tumour line TR). Both tumour lines grew vigorously never expressing any morphogenic potential. In this study, cactus shoots, callus, normal and hyperhydric regenerants and tumours (TW and TR) were compared with regard to peroxidase (EC1.11.1.7) and esterase (arylesterase EC 3.1.1.2 and carboxylesterase EC 3.1.1.1.) activity and isoenzyme patterns. Lowest guaiacol peroxidase activity was measured in the cactus shoots and in the normal regenerants. Callus, hyperhydric regenerants and tumours had 6 to 7 times higher peroxidase activity than the shoots. Esterase activity was measured with 1- and 2-naphtylacetate as broad-spectrum substrates. Highest esterase activity was determined in tumours with both substrates. All tissues, except the TR tumour, had higher esterase activity for 2- than for 1-naphtylacetate. Peroxidase and esterase isoenzyme patterns were not completely identical between the investigated tissues. Unorganised callus and tumours differed from the shoots and normal regenerants.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA