Pregled bibliografske jedinice broj: 1227437
CFD modelling of the continously operated microextraction of proteins – a shortcut to new microextractor designes
CFD modelling of the continously operated microextraction of proteins – a shortcut to new microextractor designes // Book of abstracts, The 4th International Congress on “Green Extraction of Natural Products” (GENP2022) / Karlović, Sven ; Dujmić, Filip ; Ninčević Grassino, Antonela (ur.).
Zagreb: Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 2022. str. 16-16 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1227437 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
CFD modelling of the continously operated
microextraction of proteins – a shortcut to new
microextractor designes
Autori
Jurinjak Tušek, Ana ; Valinger, Davor ; Šalić, Anita ; Zelić, Bruno
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of abstracts, The 4th International Congress on “Green Extraction of Natural Products” (GENP2022)
/ Karlović, Sven ; Dujmić, Filip ; Ninčević Grassino, Antonela - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 2022, 16-16
ISBN
978-953-6893-16-4
Skup
The 4th International Congress on “Green Extraction of Natural Products” (GENP2022)
Mjesto i datum
Poreč, Hrvatska, 27.10.2022. - 28.10.2022
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
protein extraction ; aqueous two-phase systems (ATPS) ; microextractor ; CFD
Sažetak
Aqueous Two-Phase Systems (ATPSs) have attracted much attention due to their ability to separate and purify various biological molecules with high yield and purity. ATPS can be used for separation and purification depending on their composition. ATPSa are mostly polymer-polymer or polymer-salt systems, but recently Deep Eutectic Solvents (DESs) have also been used for ATPS separations because they are more sustainable and cost- effective as solvents than polymers. To develop an efficient extraction process, the geometry of the extractor and the operating conditions should be optimized in addition to the composition of the extraction solvent. Due to the high surface-to- volume ratio, higher heat and mass transfer rates, and short diffusion path, continuously operated microextractors are widely used to improve mass transfer between two phases. In this work, ATPS composed of K2HPO4 and betaine:urea DES for lipase purification and ATPS composed of K2HPO4 and choline chloride:poly(ethylene glycol):zinc chloride DES for laccase purification in continuously operated microextractors were analyzed using Computational Fluid Dynamics (CFD). CFD simulations were performed to illustrate and visualize the interactions between two phases during extraction. The CFD simulations of the ATPS flow profiles in the microextractor were performed using COMSOL Multyphysics v.4.2 finite element software. A laminar flow model with Lagrangian specification of the field and Ditrich- type boundary conditions were used. CFD simulations were performed by estimating the fluid properties assuming simple mass balances. The results can be used to determine the position of the enzyme separation in the microchannel. Based on the obtained results, new microextractor configurations can be proposed that would enhance extraction efficiency.
Izvorni jezik
Engleski
Znanstvena područja
Kemijsko inženjerstvo, Interdisciplinarne tehničke znanosti, Biotehnologija
POVEZANOST RADA
Projekti:
UNESCO--4500461754 - Kontinuirana ekstrakcija proteina u mikroekstraktoru vodenim dvofaznim sustavima na bazi prirodnih eutektičkih otapala: od otpada do vrijednog proizvoda (Šalić, Anita, UNESCO ) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Fakultet kemijskog inženjerstva i tehnologije, Zagreb,
Sveučilište Sjever, Koprivnica