Naslov
Expression and identification of transcription factors involved in postnatal cortical development in opossum (Monodelphis domestica)

Autori
Ban, Jelena ; Baričević, Zrinko ; Tomljanović, Ivana ; Mladinić, Miranda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of Genetika 2022 : 9th Congress of the Genetic Society of Slovenia and 9th meeting of the Slovenian Society for Human Genetics with international participation / Zorc, Minja ; Dovč, Peter - Ljubljana : Genetic Society of Slovenia, 2022, 66-66

ISBN
978-961-93545-7-5

Skup
9th Congress of the Genetic Society of Slovenia

Mjesto i datum
Ljubljana, Slovenija, 28.09.2022. - 30.09.2022

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
neurodevelopment ; neuroregeneration ; cortex ; transcription factors ; biomarkers

Sažetak
The South American grey short-tailed opossum (Monodelphis domestica) provides an excellent opportunity to investigate mammalian central nervous system (CNS) development and regeneration. Unlike other mammals, marsupials including M. domestica can fully functionally recover their spinal cord after injury during the first two postnatal weeks. Neurodegeneration can be also explored, since the origin of many late-onset neurodegenerative diseases is linked to early development. The M. domestica genome contains 18.000-20.000 protein-coding genes, with the majority (~96.7%) having an eutherian homologue. We have recently performed proteome analysis by mass spectrometry obtaining differentially expressed proteins identified in opossum spinal cords during the critical period of development when regeneration stops being possible (postnatal days (P)5 and P18, respectively). Interestingly, several proteins related to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) as well as Alzheimer's, Parkinson and Huntignton diseases were identified. To further investigate developmental processes involved in postnatal opossum CNS, we have examined the expression of transcription factors such as SOX2 and SOX9 involved in neural stem cell (NSC) differentiation during postnatal cortical neuro- and gliogenesis using isotropic fractionator method on P5, P18 and P30 opossums. Surprisingly, coexpression of both stem cell (SOX2) and neuronal markers (NeuN) were confirmed in significant number (56%) of cells throughout the first postnatal month. Immunohistochemistry analysis on cortical slices confirmed the presence of double- positive SOX2/NeuN cells as well as coexpression of SOX2/SOX9 in ventricular zone from which NeuN- positive neurons migrate and ultimately develop six-layered cortical structure. In addition to expression in NSC in ventricular zone, SOX9 in P18 and P30 cortices becomes exclusively expressed in astrocytes, while SOX2 coexpression in neurons indicates its putative role during opossum cortical neurogenesis. M. domestica therefore offer a novel model organism for a variety of comparative and developmental studies as well as new insights into CNS re- and degeneration.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA