Pregled bibliografske jedinice broj: 1226678
In vitro regeneration of healthy Croatian autochthonous grapevine clones for repository clones establishment
In vitro regeneration of healthy Croatian autochthonous grapevine clones for repository clones establishment // Book of Abstracts IV International Symposium for Agriculture and Food
Skopje: Faculty of Agricultural Sciences and Food, 2022. str. 181-181 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1226678 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
In vitro regeneration of healthy Croatian autochthonous grapevine clones for repository clones establishment
Autori
Marković, Zvjezdana ; Preiner, Darko ; Šikuten, Iva ; Štambuk, Petra ; Tomaz, Ivana ; Karoglan Kontić, Jasminka ; Maletić, Edi ; Stupić, Domagoj ; Andabaka, Željko ; Rendulić, Nera
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts IV International Symposium for Agriculture and Food
/ - Skopje : Faculty of Agricultural Sciences and Food, 2022, 181-181
ISBN
13978-9989-76-5
Skup
4th International Symposium of Agriculture and Food (ISAF 2022)
Mjesto i datum
Ohrid, Sjeverna Makedonija, 12.10.2022. - 14.10.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
in vitro regeneration, autochthonous clones, grapevine repository, tissue culture establishment
Sažetak
Grapevine represents a significant crop in agricultural production in Croatia. Intensive work has been made on the Croatian autochthonous cultivars in the last two decades. A clone repository of autochthonous cultivars in virus-free conditions is safe and a legally regulated conservatory method of a specific crop's gene base. Considering different geographical origin of initial material, accessions can be multiplied in tissue culture presenting a much easier way than the classical propagation and ensuring a healthy plant material. The trial has covered 27 registered clones from 9 Croatian autochthonous cultivars. The material for in vitro establishment were 10 shoot tips from one vine of each clone. Vines are part of in situ mother blocks with the highest sanitary cleanliness. Material was taken in two periods, before and after flowering phenophase. Collected shoot tips were inoculated on a medium (Murashige and Skoog, 1 mg/l 6-benzylaminopurine + 0, 05 mg/l 3-indol-acetic acid). Regeneration of shoot tips was recorded after 6 weeks. Mature in vitro plants were subjected to acclimatization. Results achieved were in a range between 10-100% and the average percentage of regeneration is 30-40% in both periods. These preliminary research trials confirm the necessity for more detailed testing of tissue culture protocols for each cultivar of interest.
Izvorni jezik
Engleski
Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0005 - Znanstveni centar izvrsnosti za bioraznolikost i molekularno oplemenjivanje bilja (ZCI CroP-BioDiv) (Šatović, Zlatko; Liber, Zlatko) ( CroRIS)
Ustanove:
Agronomski fakultet, Zagreb,
Sveučilište u Zagrebu
Profili:
Zvjezdana Marković
(autor)
Jasminka Karoglan-Kontić
(autor)
Iva Šikuten
(autor)
Petra Štambuk
(autor)
Edi Maletić
(autor)
Željko Andabaka
(autor)
Domagoj Stupić
(autor)
Ivana Tomaz
(autor)
Darko Preiner
(autor)