Naslov
The influence of extraction and enviromental parameters on the isolation of bioactive compounds from nettle (Urtica dioica L.)

Autori
Cegledi, Ena ; Repajić, Maja ; Elez Garofulić, Ivona ; Zorić, Zoran ; Radman, Sanja ; Dragović-Uzelac, Verica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 4th International scientific and expert conference “Natural resources, green technology and sustainable development/4-GREEN2022“ / Radojčić Redovniković, I. ; Jakovljević, T. ; Stojaković, R. ; Erdec, D. ; Damjanović, A. - Zagreb : Hendrih Feldbauer, 2022, 104-104

Skup
4th Natural resources, green technology and sustainable development/4 (GREEN2022)

Mjesto i datum
Zagreb, Hrvatska, 14.09.2022. - 16.09.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Urtica dioica L., green extraction, bioactive molecules, phenological stage, ha

Sažetak
Nettle (Urtica dioica L.) is one of the medicinal plant species that, due to its chemical com- position and content of bioactive components, is an excellent basis for obtaining products with high biological potential. It is a perennial wild plant, known in folk medicine since ancient times, widely distributed and adapted to different climate zones. Nettle products are mostly used in industry as liquid extracts, so it is important to apply and optimize a suitable extraction method to obtain higher yields and ensure the stability of targeted bioactive molecules during extraction. Therefore, various advanced extraction techniques such as microwave assisted extraction (MAE) and accelerated solvent extraction (ASE) were used throughout this study and compared with conventional techniques. One of them - ASE, as a green extraction technique, was inestigated as potential for the recovery of the antioxidant molecules (polyphenols, pigments and phytosterols) from all parts of the nettle (leaves, stalks, roots). Also, changes in chemical composition and distribution of compounds depend on the climate and habitat of the plant and can occur as the plant matures, with bioactive molecules being present in different ratio during different phenological stages. So, the aim of this study was to optimize the extraction of bioactive molecules from nettle using ASE and to examine the influence of phenological stage and habitat on their accumulation during vegetation. The optimal conditions for the extraction of polyphenols and pigments from nettle leaf extract were 110 °C, 10 min of static time and three or four extraction cycles. On the other hand, for the efficient isolation of phytoserols from nettle root extract, the optimal condiotions were 150 °C, 10 min of static time during four extraction cycles. Furthermore, ASE showed better performance in comparison with conventional extraction techniques. In addition, comparing the proportion of bioactive molecules in the leaves and stalks of nettle, more natural antioxidants were accumulated in the leaves and should be collected during the early phenological period (before and at the flowering stage). Moreover, the amounts of polyphenols and pigments from nettle greatly differed based on the natural habitat, as samples from the seaside region were characterized with elevated accumulation of pigments, while higher polyphenols amounts were present in habitats located in continental and mountain areas.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA