Naslov
TKIVNO BANKARSTVO: OBRADA SPONGIOZNOG KOŠTANOG TKIVA DOBIVENOG IZ GLAVE FEMURA ŽIVIH DARIVATELJA
(TISSUE BANKING: PROCESSING OF SPONGY BONE OBTAINED FROM THE FEMORAL HEAD OF LIVING DONORS)

Autori
Puljić, Dominik ; Zekušić, Marija ; Vučemilo, Tiha ; Jularić, Anamarija ; Skoko, Marija ; Bujić Mihica, Marina ; Vrgoč Zimić, Ivana ; Dolenec, Tamara ; Vidović, Dinko ; Babić, Slaven ; Batarilo, Ivanka ; Ramić, Snježana ; Sesar, Patricija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka (Hrvatski biološki kongres s međunarodnim sudjelovanjem) / - , 2022, 260-261

Skup
14. Hrvatski biološki kongres

Mjesto i datum
Pula, Hrvatska, 12.10.2022. - 16.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
spongiozno koštano tkivo, mikrobiološka sterilnost, patohistološka analiza
(spongy bone tissue, microbiological sterility, pathohistological analysis)

Sažetak
Spongy bone is used in reconstructive procedures as a valuable material for bone regeneration and replacement. The aim was to examine the aseptic procurement and cutting of femoral head fragments in the operating room, the sterility of spongy bone obtained in the microbiological safety cabinet (MSC) and to show histological structure and vitality of tissue. During the implantation of a total hip endoprosthesis in living donors, the femoral heads were removed and cut into fragments. A swab for microbiological and a biopsy for pathohistological analysis were sampled from each head. Fragments were processed in MSC and the sterility of spongiosis was controlled by analysing duplicate swabs and biopsies by direct inoculation in a liquid medium for anaerobic and aerobic bacteria and fungi. Sterility of environmental conditions (surface, air and operator’s fingers) was controlled by contact and sediment plates. 27 samples were microbiologically analysed: 16 from the operating room (biological n=10, environmental n=6) and 11 from MSC (biological n=7, environmental n=4). A 100 % sterile result of bone tissue and operator's fingers was obtained in the operating room and the results of air control were within acceptable limits. All results sampled in the MSC were 100 % sterile. Pathohistological analysis confirmed vital osteocytes in the lacunae of the circumferential lamellae, individual osteoclasts and numerous osteoblasts in the surface of the bone without any pathological changes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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