Naslov
Whole Genome Characterization of the unusual Rotavirus A strains with zoonotic background detected in hospitalized children in Croatia

Autori
Kunić, Valentina ; Mikuletič, Tina ; Steyer, Andrej ; Kogoj, Rok ; Šoprek, Silvija ; Tešović, Goran ; Konjik, Vlatka ; Jukić Guć, Jelena ; Krželj, Vjekoslav ; Krešić, Nina ; Brnić, Dragan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14th International dsRNA Virus Symposium / - , 2022

Skup
14th International dsRNA Virus Symposium

Mjesto i datum
Banff, Kanada, 10.10.2022. - 14.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Rotavirus A, hospitalized children, diarrhea, whole genome sequencing, zoonotic transmission

Sažetak
Rotavirus A (RVA) belongs among the leading causes of gastroenteritis in young children and animals, varying in severity from mild to deadly. Rotavirus genome consists of 11-segmented, double-stranded RNA. We aimed to determine the complete genome constellations of the unusual RVA genotypes discovered in Croatian children hospitalized with acute gastroenteritis collected during seasons 2018 to 2020. Following multiplex RT-PCR and Sanger sequencing, samples with host-unusual genotypes were singled out for the Illumina-based whole-genome sequencing. Attained constellations for samples D230-ZG, D329-OB, D248-ZG, and D399-SD were G4-P[6]-I1-R1-C1-M1-A1-N1-T7-E1-H1, G4-P[6]- I1-R1-C1-M1-A1-N1-T1-E1-H1, G6-P[6]-I2-R2-C2-M2- A3-N2-T6-E2-H3, G8-P[14]-I2-R2-C2-M2-A3-N2-T6-E2- H3, respectively. Two G4P[6] strains presented with a Wa-like constellation that evolutionary shares its backbone with porcine RVAs, except for the human-originated NSP1 and NSP2 segments. Conversely, strains G4P[6] and G8P[14] displayed a bovine-like genotype constellation. These two strains revealed a very close evolutionary relationship in all segments, some to the clade level, with the RVAs from the Artiodactyla family, hence a plausible direct zoonotic transmission could be hypothesized. Despite the relatedness with the Artiodactyla, it is interesting to note that during concurrent One Health research, the P[14] genotype within the G8P[14] human RVA strain, was detected only in the Croatian wild canine population. RVA strains with zoonotic background, currently detected sporadically, could be more present in the overall human population, eluding the present study by causing clinically milder, rarely hospitalized cases. Therefore, future surveillance is essential to fully determine the influence the wildlife and domestic animal populations have on the epidemiology of the specified RVA strains circulating in humans.

Izvorni jezik
Engleski

POVEZANOST RADA