Pregled bibliografske jedinice broj: 1223486
Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure
Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure // PLOS Genetics, 18 (2022), 10; 1-26 doi:10.1371/journal.pgen.1010432 (međunarodna recenzija, članak, znanstveni)
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Naslov
Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure
Autori
Novačić, Ana ; Menéndez, Dario ; Ljubas, Jurica ; Barbarić, Slobodan ; Stutz, Françoise ; Soudet, Julien ; Stuparević, Igor
Izvornik
PLOS Genetics (1553-7404) 18
(2022), 10;
1-26
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
antisense transcription, RNA exosome, PHO5, chromatin, yeast
Sažetak
Pervasive transcription of eukaryotic genomes generates non-coding transcripts with regulatory potential. We examined the effects of non-coding antisense transcription on the regulation of expression of the yeast PHO5 gene, a paradigmatic case for gene regulation through promoter chromatin remodeling. A negative role for antisense transcription at the PHO5 gene locus was demonstrated by leveraging the level of overlapping antisense transcription through specific mutant backgrounds, expression from a strong promoter in cis, and use of the CRISPRi system. Furthermore, we showed that enhanced elongation of PHO5 antisense leads to a more repressive chromatin conformation at the PHO5 gene promoter, which is more slowly remodeled upon gene induction. The negative effect of antisense transcription on PHO5 gene transcription is mitigated upon inactivation of the histone deacetylase Rpd3, showing that PHO5 antisense RNA acts via histone deacetylation. This regulatory pathway leads to Rpd3-dependent decreased recruitment of the RSC chromatin remodeling complex to the PHO5 gene promoter upon induction of antisense transcription. Overall, the data in this work reveal an additional level in the complex regulatory mechanism of PHO5 gene expression by showing antisense transcription-mediated repression at the level of promoter chromatin structure remodeling.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
UIP-2017-05-4411 - In silico i in vivo analiza transkriptoma stanične stijenke kvasca Saccharomyces cerevisiae i primjena dobivenih rezultata u konstrukciji novih biotehnoloških sojeva (SCCWTRANS) (Stuparevic, Igor, HRZZ - 2017-05) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
- Nature Index