Pregled bibliografske jedinice broj: 1223285
Mouse Models for Cytomegalovirus Infections in Newborns and Adults
Mouse Models for Cytomegalovirus Infections in Newborns and Adults // Current Protocols, 2 (2022), 9; 1-23 doi:10.1002/cpz1.537 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1223285 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Mouse Models for Cytomegalovirus Infections in
Newborns and Adults
Autori
Brizić, Ilija ; Lisnić, Berislav ; Krstanović, Fran ; Brune, Wolfram ; Hengel, Hartmut ; Jonjić, Stipan
Izvornik
Current Protocols (2691-1299) 2
(2022), 9;
1-23
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
cytomegalovirus infections ; disease models ; fibroblasts ; herpesviridae infections ; MCMV ; mice ; mouse cytomegalovirus ; salivary gland ; viral load ; viral plaque assay ; virology ; virus replication
Sažetak
His article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-PZS-2019-02-7879 - Biologija citomegalovirusne infekcije u mozgu tijekom razvoja i u latenciji (CMVbrain) (Brizić, Ilija, HRZZ ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Citiraj ovu publikaciju:
Časopis indeksira:
- Scopus
- MEDLINE
