Pregled bibliografske jedinice broj: 1222836
Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars
Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars // Plants, 11 (2022), 11; 1485, 14 doi:10.3390/plants11111485 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1222836 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Virus and Virus-like Pathogens in the Grapevine
Virus Collection of Croatian Autochthonous
Grapevine Cultivars
Autori
Vončina, Darko ; Diaz-Lara, Alfredo ; Preiner, Darko ; Al Rwahnih, Maher ; Stevens, Kristian ; Jurić, Snježana ; Malenica, Nenad ; Šimon, Silvio ; Meng, Baozhong ; Maletić, Edi ; Fulgosi, Hrvoje ; Cvjetković, Bogdan
Izvornik
Plants (2223-7747) 11
(2022), 11;
1485, 14
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
grapevine virus ; viroid ; “flavescence dorée” ; ELISA ; Western blot ; RT-PCR ; cloning ; sequencing ; LAMP
Sažetak
Grapevine collections play an important role, especially in the study of viruses and virus-like pathogens. In 2009, after an initial ELISA screening for eight viruses (arabis mosaic virus, grapevine fanleaf virus, grapevine fleck virus, grapevine leafroll-associated viruses 1, 2, and 3, and grapevine viruses A and B), a collection of 368 grapevine accessions representing 14 different Croatian autochthonous cultivars and containing single or mixed infection of viruses was established to further characterize the viral pathogens. Subsequently, Western blot, RT-PCR, cloning, and sequencing revealed that grapevine rupestris stem pitting-associated virus was frequently found in accessions of the collection, with isolates showing substantial genetic diversity in the helicase and coat protein regions. High-throughput sequencing of 22 grapevine accessions provides additional insight into the viruses and viroids present in the collection and confirms the fact that Croatian autochthonous grapevine cultivars have high infection rates and high virome diversity. The recent spread of “flavescence dorée” phytoplasma in Europe has not spared the collection. After the first symptoms observed in 2020 and 2021, the presence of phytoplasma was confirmed by LAMP in six grapevine accessions and some of them were lost. Single or multiple viruses and viroids, as well as own rooted grapevines in the collection, make the plants susceptible to various abiotic factors, which, together with the recent occurrence of “flavescence dorée”, makes the maintenance of the collection a challenge. Future efforts will be directed towards renewing the collection, as 56% of the original collection has been lost in the last 13 years.
Izvorni jezik
Engleski
Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti
POVEZANOST RADA
Projekti:
IP-2018-01-1305 - Ekologija i karakterizacija dva nova virusa vinove loze (ENVISaGE) (Voncina, Darko, HRZZ - 2018-01) ( CroRIS)
EK-EFRR-KK.01.1.1.04.0031 - Novi početak za stare hrvatske sorte vinove loze (CroVitiRestart) (Preiner, Darko; Ašperger, Danijela; Kovačević-Ganić, Karin, EK - KK.01.1.1.04) ( CroRIS)
MZOS-178-1781844-2758 - Unutarsortna raznolikost vinove loze i unaprjeđenje klonske selekcije (Maletić, Edi, MZOS ) ( CroRIS)
MZOS-178-1781844-2692 - Identifikacija fitopatogena molekularnim i drugim metodama (Cvjetković, Bogdan, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Agronomski fakultet, Zagreb
Profili:
Darko Vončina
(autor)
Nenad Malenica
(autor)
Snježana Jurić
(autor)
Bogdan Cvjetković
(autor)
Hrvoje Fulgosi
(autor)
Edi Maletić
(autor)
Silvio Šimon
(autor)
Darko Preiner
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus