Pregled bibliografske jedinice broj: 1222805
Detection, Transmission, and Characterization of Grapevine Virus H in Croatia
Detection, Transmission, and Characterization of Grapevine Virus H in Croatia // Pathogens, 10 (2021), 12; 1578, 11 doi:10.3390/pathogens10121578 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1222805 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Detection, Transmission, and Characterization of
Grapevine Virus H in Croatia
Autori
Jagunić, Martin ; Lazarević, Boris ; Nikolić, Kristina ; Stupić, Domagoj ; Preiner, Darko ; Vončina, Darko
Izvornik
Pathogens (2076-0817) 10
(2021), 12;
1578, 11
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
RT-PCR ; vine mealybug ; coat protein ; RdRP ; sequencing
Sažetak
A survey of recently discovered vitiviruses was performed on 113 Croatian autochthonous grapevine cultivars from the national collection “Jazbina” using one-step RT-PCR. The presence of grapevine virus H (GVH) was confirmed in nine (7.9%) cultivars and grapevine virus G in eight (7.1%), while the presence of grapevine viruses I and J were not detected. GVH was transmitted by the vine mealybug (Planococcus ficus) from a source plant to grapevine seedlings with a 10.5% transmission rate using a combination of 10 first and second instars per plant with 48 and 72 h of acquisition and inoculation access period, respectively. Transmission correlated with the presence of grapevine leafroll-associated virus 3 (GLRaV-3) in the GVH-source plant and recipient seedlings. No alternative GVH host was identified. A comparison of 356 nt fragments of the RdRP and CP coding regions showed nucleotide identity between the Croatian GVH isolates in the range of 95.5–99.2% and 97.5–99.4% and amino acid identity between 95.8 and 100% and between 98.3 and 100%, respectively. Comparison with foreign isolates revealed nucleotide sequence similarity in the RdRP and CP between 94 and 100% and between 97.7– 100%, respectively. To the best of our knowledge, this is the first report of GVH in Croatia and the first identification of the vine mealybug as a vector of GVH.
Izvorni jezik
Engleski
Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti
POVEZANOST RADA
Projekti:
IP-2018-01-1305 - Ekologija i karakterizacija dva nova virusa vinove loze (ENVISaGE) (Voncina, Darko, HRZZ - 2018-01) ( CroRIS)
EK-EFRR-KK.01.1.1.04.0031 - Novi početak za stare hrvatske sorte vinove loze (CroVitiRestart) (Preiner, Darko; Ašperger, Danijela; Kovačević-Ganić, Karin, EK - KK.01.1.1.04) ( CroRIS)
Ustanove:
Agronomski fakultet, Zagreb
Profili:
Darko Vončina
(autor)
Domagoj Stupić
(autor)
Martin Jagunić
(autor)
Darko Preiner
(autor)
Boris Lazarević
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus