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Pregled bibliografske jedinice broj: 1222805

Detection, Transmission, and Characterization of Grapevine Virus H in Croatia


Jagunić, Martin; Lazarević, Boris; Nikolić, Kristina; Stupić, Domagoj; Preiner, Darko; Vončina, Darko
Detection, Transmission, and Characterization of Grapevine Virus H in Croatia // Pathogens, 10 (2021), 12; 1578, 11 doi:10.3390/pathogens10121578 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1222805 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Detection, Transmission, and Characterization of Grapevine Virus H in Croatia

Autori
Jagunić, Martin ; Lazarević, Boris ; Nikolić, Kristina ; Stupić, Domagoj ; Preiner, Darko ; Vončina, Darko

Izvornik
Pathogens (2076-0817) 10 (2021), 12; 1578, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
RT-PCR ; vine mealybug ; coat protein ; RdRP ; sequencing

Sažetak
A survey of recently discovered vitiviruses was performed on 113 Croatian autochthonous grapevine cultivars from the national collection “Jazbina” using one-step RT-PCR. The presence of grapevine virus H (GVH) was confirmed in nine (7.9%) cultivars and grapevine virus G in eight (7.1%), while the presence of grapevine viruses I and J were not detected. GVH was transmitted by the vine mealybug (Planococcus ficus) from a source plant to grapevine seedlings with a 10.5% transmission rate using a combination of 10 first and second instars per plant with 48 and 72 h of acquisition and inoculation access period, respectively. Transmission correlated with the presence of grapevine leafroll-associated virus 3 (GLRaV-3) in the GVH-source plant and recipient seedlings. No alternative GVH host was identified. A comparison of 356 nt fragments of the RdRP and CP coding regions showed nucleotide identity between the Croatian GVH isolates in the range of 95.5–99.2% and 97.5–99.4% and amino acid identity between 95.8 and 100% and between 98.3 and 100%, respectively. Comparison with foreign isolates revealed nucleotide sequence similarity in the RdRP and CP between 94 and 100% and between 97.7– 100%, respectively. To the best of our knowledge, this is the first report of GVH in Croatia and the first identification of the vine mealybug as a vector of GVH.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti



POVEZANOST RADA


Projekti:
IP-2018-01-1305 - Ekologija i karakterizacija dva nova virusa vinove loze (ENVISaGE) (Voncina, Darko, HRZZ - 2018-01) ( CroRIS)
EK-EFRR-KK.01.1.1.04.0031 - Novi početak za stare hrvatske sorte vinove loze (CroVitiRestart) (Preiner, Darko; Ašperger, Danijela; Kovačević-Ganić, Karin, EK - KK.01.1.1.04) ( CroRIS)

Ustanove:
Agronomski fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Jagunić, Martin; Lazarević, Boris; Nikolić, Kristina; Stupić, Domagoj; Preiner, Darko; Vončina, Darko
Detection, Transmission, and Characterization of Grapevine Virus H in Croatia // Pathogens, 10 (2021), 12; 1578, 11 doi:10.3390/pathogens10121578 (međunarodna recenzija, članak, znanstveni)
Jagunić, M., Lazarević, B., Nikolić, K., Stupić, D., Preiner, D. & Vončina, D. (2021) Detection, Transmission, and Characterization of Grapevine Virus H in Croatia. Pathogens, 10 (12), 1578, 11 doi:10.3390/pathogens10121578.
@article{article, author = {Jaguni\'{c}, Martin and Lazarevi\'{c}, Boris and Nikoli\'{c}, Kristina and Stupi\'{c}, Domagoj and Preiner, Darko and Von\v{c}ina, Darko}, year = {2021}, pages = {11}, DOI = {10.3390/pathogens10121578}, chapter = {1578}, keywords = {RT-PCR, vine mealybug, coat protein, RdRP, sequencing}, journal = {Pathogens}, doi = {10.3390/pathogens10121578}, volume = {10}, number = {12}, issn = {2076-0817}, title = {Detection, Transmission, and Characterization of Grapevine Virus H in Croatia}, keyword = {RT-PCR, vine mealybug, coat protein, RdRP, sequencing}, chapternumber = {1578} }
@article{article, author = {Jaguni\'{c}, Martin and Lazarevi\'{c}, Boris and Nikoli\'{c}, Kristina and Stupi\'{c}, Domagoj and Preiner, Darko and Von\v{c}ina, Darko}, year = {2021}, pages = {11}, DOI = {10.3390/pathogens10121578}, chapter = {1578}, keywords = {RT-PCR, vine mealybug, coat protein, RdRP, sequencing}, journal = {Pathogens}, doi = {10.3390/pathogens10121578}, volume = {10}, number = {12}, issn = {2076-0817}, title = {Detection, Transmission, and Characterization of Grapevine Virus H in Croatia}, keyword = {RT-PCR, vine mealybug, coat protein, RdRP, sequencing}, chapternumber = {1578} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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