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Pregled bibliografske jedinice broj: 1222775

Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia


Jagunić, Martin; Diaz-Lara, Alfredo; Rwahnih, Maher Al; Preiner, Darko; Stevens, Kristian; Zdunić, Goran; Hwang, Minsook; Vončina, Darko
Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia // Plants, 11 (2022), 16; 2135, 14 doi:10.3390/plants11162135 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1222775 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia

Autori
Jagunić, Martin ; Diaz-Lara, Alfredo ; Rwahnih, Maher Al ; Preiner, Darko ; Stevens, Kristian ; Zdunić, Goran ; Hwang, Minsook ; Vončina, Darko

Izvornik
Plants (2223-7747) 11 (2022), 16; 2135, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
conventional PCR ; real-time PCR ; autochthonous and introduced grapevine varieties ; sequencing ; phylogeny

Sažetak
Grapevine badnavirus 1 (GBV-1) was recently discovered in grapevine using high throughput sequencing. In order to carry out large-scale testing that will allow for better insights into virus distribution, conventional and real-time PCR assays were developed using sequences both from previously known, and four newly characterized isolates. Throughout the growing season and dormancy, GBV-1 can be detected by real-time PCR using available tissue, with the possibility of false-negative results early in vegetation growth. GBV-1 real-time PCR analysis of 4302 grapevine samples from the Croatian continental and coastal wine-growing regions revealed 576 (~13.4%) positive vines. In the continental wine-growing region, virus incidence was confirmed in only two collection plantations, whereas in the coastal region, infection was confirmed in 30 commercial vineyards and one collection plantation. Infection rates ranged from 1.9 to 96% at the different sites, with predominantly autochthonous grapevine cultivars infected. Conventional PCR products obtained from 50 newly discovered GBV-1 isolates, containing the 375 nucleotides long portion of the reverse transcriptase gene, showed nucleotide and amino acid identities ranging from 94.1 to 100% and from 92.8 to 100%, respectively. The reconstructed phylogenetic tree positioned the GBV-1 isolates taken from the same vineyard close to each other indicating a possible local infection event, although the tree nodes were generally not well supported.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti



POVEZANOST RADA


Projekti:
IP-2018-01-1305 - Ekologija i karakterizacija dva nova virusa vinove loze (ENVISaGE) (Voncina, Darko, HRZZ - 2018-01) ( CroRIS)

Ustanove:
Agronomski fakultet, Zagreb

Profili:

Avatar Url Darko Vončina (autor)

Avatar Url Goran Zdunić (autor)

Avatar Url Martin Jagunić (autor)

Avatar Url Darko Preiner (autor)

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Jagunić, Martin; Diaz-Lara, Alfredo; Rwahnih, Maher Al; Preiner, Darko; Stevens, Kristian; Zdunić, Goran; Hwang, Minsook; Vončina, Darko
Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia // Plants, 11 (2022), 16; 2135, 14 doi:10.3390/plants11162135 (međunarodna recenzija, članak, znanstveni)
Jagunić, M., Diaz-Lara, A., Rwahnih, M., Preiner, D., Stevens, K., Zdunić, G., Hwang, M. & Vončina, D. (2022) Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia. Plants, 11 (16), 2135, 14 doi:10.3390/plants11162135.
@article{article, author = {Jaguni\'{c}, Martin and Diaz-Lara, Alfredo and Rwahnih, Maher Al and Preiner, Darko and Stevens, Kristian and Zduni\'{c}, Goran and Hwang, Minsook and Von\v{c}ina, Darko}, year = {2022}, pages = {14}, DOI = {10.3390/plants11162135}, chapter = {2135}, keywords = {conventional PCR, real-time PCR, autochthonous and introduced grapevine varieties, sequencing, phylogeny}, journal = {Plants}, doi = {10.3390/plants11162135}, volume = {11}, number = {16}, issn = {2223-7747}, title = {Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia}, keyword = {conventional PCR, real-time PCR, autochthonous and introduced grapevine varieties, sequencing, phylogeny}, chapternumber = {2135} }
@article{article, author = {Jaguni\'{c}, Martin and Diaz-Lara, Alfredo and Rwahnih, Maher Al and Preiner, Darko and Stevens, Kristian and Zduni\'{c}, Goran and Hwang, Minsook and Von\v{c}ina, Darko}, year = {2022}, pages = {14}, DOI = {10.3390/plants11162135}, chapter = {2135}, keywords = {conventional PCR, real-time PCR, autochthonous and introduced grapevine varieties, sequencing, phylogeny}, journal = {Plants}, doi = {10.3390/plants11162135}, volume = {11}, number = {16}, issn = {2223-7747}, title = {Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia}, keyword = {conventional PCR, real-time PCR, autochthonous and introduced grapevine varieties, sequencing, phylogeny}, chapternumber = {2135} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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