Naslov
Blood cells morphology and cytochemistry of captive Utila iguana (Ctenosaura bakeri)

Autori
Belić, Maja ; Faraguna, Siniša ; Turk, Romana ; Robić, Mirna ; Cizelj, Ivan ; Lukač, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme and Proceedings ESVCP 2022 ECVCP / - , 2022, 153-154

Skup
24th ESVCP/ECVCP Congress

Mjesto i datum
Beograd, Srbija, 05.10.2022. - 08.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Utila iguana, hematology, cytochemistry of blood cells, endangered species

Sažetak
Background. Utila iguana (Ctenosaura bakeri) is a highly protected, endangered and rare animal species which has only one natural habitat left. Because of that, there are no existing researches on the blood diagnostic parameters of this species. Objectives. The aim was to evaluate morphologic and cytochemical features of blood cells in clinically healthy Utila iguanas. Material and Methods. Blood was collected from ten adult Utila iguanas from the Zagreb City Zoo. For each animal four blood films were made. The blood films were air-dried and two films of each animal were stained with a modified Wright Giemsa stain, while the other two films were used for cytochemical staining with periodic acid-Shiff (PAS). PAS staining was used to distinguish lymphocytes from thrombocytes and heterophils from eosinophils. Results. On the Wright Giemsa stained blood films morphologic features of erythrocytes corresponded to the erythrocytes of reptiles described in the literature. Heterophils were also clearly recognizable, while differentiating small lymphocytes from thrombocytes, was sometimes difficult. On PAS stained blood films, lymphocytes stained negative while thrombocytes stained positive which helped differentiation between these cells. Heterophils and monocytes were also PAS positive and eosinophils and basophils were PAS negative. Conclusion. The data obtained in this study determine blood cells morphology and cytochemistry of clinically healthy Utila iguanas, which contributes to a better knowledge of hematology of these animals which is an important factor in preserving this rare species.

Izvorni jezik
Engleski

POVEZANOST RADA