Naslov
Possible role of SSB protein in the regulation of antibiotic production

Autori
Pipalović, Goran ; Filić, Želimira ; Ćehić, Mirsada ; Paradžik, Tina ; Crnolatac, Ivo ; Zahradka, Ksenija ; Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Summer school in applied molecular microbiology on microbial specialised metabolites

Mjesto i datum
Dubrovnik, Hrvatska, 10.09.2022. - 17.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Proteins ; Motifs ; Antibiotic

Sažetak
Single-strand DNA-binding (SSB) proteins have a key role in all processes of DNA metabolism. They act as recruitment platform for many genome maintenance proteins, and while doing so, they modulate their activities. Although many bacteria have additional copies of ssb genes, their biological role is poorly understood. Streptomyces coelicolor has two paralogous SSB proteins SsbA and SsbB. Our group previously found that SsbA is essential while SsbB participates in chromosomal segregation during sporulation. Our studies showed that paralogous SSB proteins possibly make interactions with various regulatory proteins. Potential interactants were found with Tandem affinity purification (TAP) and Bacterial two-hybrid (B2H) method. Literature data show that the acidic C-tip of SSB protein is important for interactions with other proteins, as well as for ssDNA binding. In order to analyze whether (or how) the altered C- terminus of paralogous SSB proteins will interfere their function, we performed various mutations in Cterminus of SsbA and SsbB proteins. Binding affinity of wild type and various mutants for ssDNA was assessed with isotermal titration calorimetry (ITC).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA