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Pregled bibliografske jedinice broj: 1221190

INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM


Mrša, Vladimir; Matičević, Ana; Lozančić, Mateja; Žunar, Bojan; Teparić, Renata; Martinić-Cezar, Tea
INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM // FEMS Conference on Microbiology: Electronic Book of Abstracts
Beograd: Federation of European Materials Societies (FEMS), 2022. str. 416-416 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 1221190 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM

Autori
Mrša, Vladimir ; Matičević, Ana ; Lozančić, Mateja ; Žunar, Bojan ; Teparić, Renata ; Martinić-Cezar, Tea

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEMS Conference on Microbiology: Electronic Book of Abstracts / - Beograd : Federation of European Materials Societies (FEMS), 2022, 416-416

ISBN
978-86-914897-8-6

Skup
2nd FEMS Conference on Microbiology

Mjesto i datum
Beograd, Srbija, 30.06.2022. - 02.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
recombinant proteins, surface display, Pir2

Sažetak
BACKGROUND Saccharomyces cerevisiae is frequently used as a host for the production of recombinant proteins. S. cerevisiae also provides the immobilization of recombinant proteins on the cell surface as an alternative to conventional immobilization methods. Yeast surface display systems are based on genetic fusion of a gene coding for a protein of interest and a gene coding for one of yeast cell wall proteins. Since immobilization of the protein through its N- or C- terminal end can affect its activity, different surface display systems were developed. Proteins of the Pir family are standardly used for N- terminal immobilization of recombinant proteins. OBJECTIVES Pir-based display systems developed so far involve fusion of the whole Pir protein with the desired protein, resulting in a large recombinant protein whose expression cause problems in its secretion and cell wall binding. To address this problem, we introduced changes in the Pir2 sequence and the protein secretion mechanisms to increase the efficiency of the Pir2- based surface display system. METHODS Using bacterial β-lactamase as a reporter enzyme, we assessed the efficiency of surface display of non-modified and modified recombinant protein Pir2bla in different yeast mutants. Amount of protein incorporated in cell wall was assessed by measurement of β-lactamase activity and by western blot. RESULTS Results show higher β-lactamase activity in strains expressing recombinant proteins containing the modified Pir2 binding region, lacking multiple internal repeats, and an increase of activity in most of the tested strains lacking activity of certain genes involved in endoplasmic reticulum-associated degradation (ERAD) pathways, or endocytosis. ACKNOWLEDGEMENTS/REFERENCES This research was funded by The Croatian Science Foundation, grant No. IP-2019-04-2891.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
IP-2019-04-2891 - Biotehnološka primjena ugradnje heterolognih proteina u stanične stijenke kvasaca (PRODIS) (Mrša, Vladimir, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

acrobat.adobe.com

Citiraj ovu publikaciju:

Mrša, Vladimir; Matičević, Ana; Lozančić, Mateja; Žunar, Bojan; Teparić, Renata; Martinić-Cezar, Tea
INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM // FEMS Conference on Microbiology: Electronic Book of Abstracts
Beograd: Federation of European Materials Societies (FEMS), 2022. str. 416-416 (poster, nije recenziran, sažetak, znanstveni)
Mrša, V., Matičević, A., Lozančić, M., Žunar, B., Teparić, R. & Martinić-Cezar, T. (2022) INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM. U: FEMS Conference on Microbiology: Electronic Book of Abstracts.
@article{article, author = {Mr\v{s}a, Vladimir and Mati\v{c}evi\'{c}, Ana and Lozan\v{c}i\'{c}, Mateja and \v{Z}unar, Bojan and Tepari\'{c}, Renata and Martini\'{c}-Cezar, Tea}, year = {2022}, pages = {416-416}, keywords = {recombinant proteins, surface display, Pir2}, isbn = {978-86-914897-8-6}, title = {INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM}, keyword = {recombinant proteins, surface display, Pir2}, publisher = {Federation of European Materials Societies (FEMS)}, publisherplace = {Beograd, Srbija} }
@article{article, author = {Mr\v{s}a, Vladimir and Mati\v{c}evi\'{c}, Ana and Lozan\v{c}i\'{c}, Mateja and \v{Z}unar, Bojan and Tepari\'{c}, Renata and Martini\'{c}-Cezar, Tea}, year = {2022}, pages = {416-416}, keywords = {recombinant proteins, surface display, Pir2}, isbn = {978-86-914897-8-6}, title = {INCREASING THE EFFICIENCY OF PIR2-BASED YEAST SURFACE DISPLAY SYSTEM}, keyword = {recombinant proteins, surface display, Pir2}, publisher = {Federation of European Materials Societies (FEMS)}, publisherplace = {Beograd, Srbija} }




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