Naslov
Nanomechanical tool for characterization of breast cancer cells

Autori
Božičević, Lucija ; Iskrzynska, Aleksandra ; Loparić, Marko ; Vinković Vrček, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, pp prezentacija, znanstveni

Izvornik
COST Action CA17140 Nano2Clinic 1st STSM Virtual Conference Book of Abstracts / Ottaviani, Maria Francesca - , 2022, 16-16

Skup
1st Conference CA17140 European Cooperation in Science and Technology (COST 2022)

Mjesto i datum
Online, 15.10.2019. - 17.10.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
atomic force microscopy ; breast cancer ; endocrine disruption

Sažetak
Some of the commonly used medicines, such as NSAIDs, antidepressants and anticonvulsives, show potential for endocrine disruption. Interplay of these drugs with endocrine hormones can affect the status of cancers, especially those of endocrine or reproductive systems. They can have proliferative effects on cells and lead to the deterioration of patient’s condition, or they can affect cancer cells negatively and therefore improve the therapy and condition. AFM is a type of scanning probe microscope and its near-field technique is based on the interaction between a sharp tip and the sample surface. This method is useful for probing the viscoelastic properties of living cells in culture and, therefore providing an indirect indicator of the structure and function of the underlying cytoskeleton and cell organelles. This method helps our understanding of cell mechanics in normal and diseased states and provides future potential in the study of disease pathophysiology and in the establishment of novel diagnostic and treatment options. The measurements were done with non-treated cells that were used as controls and the cells treated with ibuprofen in concentration of 10 μM and fluoxetine in 1 μM concentration. These compounds, as well as their concentrations were chosen for these experiments because they were shown in preliminary experiments to have estrogenic and anti-estrogenic effects. Therefore, these compounds in these concentrations affect cell proliferation of T47D-KBluc and these effects alter the mechanical properties of breast cancer cells T47D-KBluc employed in this research.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA