Naslov
Broccoli seedlings extracts change the activity of α-amylase and α-glucosidase: effect of growing temperature

Autori
Šola, Ivana ; Gulin, Silvija ; Vujčić Bok, Valerija ; Rusak, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka 7. Hrvatskog botaničog simpozija / Vuković, N. ; Šegota, V. - Zagreb : Hrvatsko botaničko društvo / Croatian Botanical Society / Vuković, N. ; Šegota, V. - Zagreb, 2022, 60-60

ISBN
978-953-8097-03-4

Skup
7. hrvatski botanički simpozij

Mjesto i datum
Zagreb, Hrvatska, 12.09.2022. - 14.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antidiabetic activity, digestive enzymes, food, global warming, microgreens

Sažetak
Plants challenged with high temperature (HT) undergo many adaptive mechanisms at molecular levels to keep normal physiological function. Such adaptations consequently influence their bioactivity. In this work, within the scope of the TEMPHYS project (IP-2020-02- 7585), we investigated the effect of HT on the antidiabetic activity of broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) seedlings. The activity was spectrophotometrically assessed via the percentage of inhibition of α-amylase and α-glucosidase, enzymes required for carbohydrate digestion. Enzyme inhibitory activity was calculated from the equation: % inhibition = 100 – [(At – Atb / Ac – Acb) x 100], where At was absorbance of test, Atb was absorbance of test blank, Ac was absorbance of control, and Acb was absorbance of control blank. Seedlings were grown in a climatic chamber with temperature range 23°C/16h, 18°C/8h for control group, and 38°C/16h, 33°C/8h for HT group. The extracts were prepared from freeze- dried plant material using boiling (100°C) water followed by incubation on rotary extractor for 1 hour at 23°C. The results showed that broccoli seedlings aqueous extracts were more efficient in the inhibition of α-amylase (88% relative to the standard acarbose), than α-glucosidase (67% relative to the standard acarbose). HT significantly (p≤0.05) decreased the potential to inhibit α-amylase (80%), however it improved the potential to inhibit α-glucosidase (70%). These results show that high growing temperature significantly changes the biological effects of plants ; moreover, their activity toward different enzymes is specifically affected. Further more detailed analyses into the effect of global warming on cruciferous (Brassicaceae) vegetables bioactivity are needed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA